A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research

Article

LCGC Europe

LCGC EuropeLCGC Europe-10-01-2004
Volume 17
Issue 10
Pages: 522–529

The guest authors describe the use of LC–MS for the analysis of peptides. They deal with peptide extraction, peptidomic analysis in organisms with and without genomic databases, identification of peptides with conserved N- and C- terminal sequences, and in situ MALDI-TOF peptide profiling.

External Link - article-128470.pdf

Articles in this issue
The Role of the Column in Preparative HPLC
Grandma's Ham
Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules
A Combination of Liquid Chromatography and Mass Spectrometry for Peptidomic Research
Recent Videos
Toby Astill | Image Credit: © Thermo Fisher Scientific
Related Content
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography

RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography

Alasdair Matheson
November 18th 2024
Article

A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).

Best of the Week: Emerging Chromatography Leader, Bladder Cancer Research, and HPLC 2025

Best of the Week: Emerging Chromatography Leader, Bladder Cancer Research, and HPLC 2025

Aaron Acevedo
November 15th 2024
Article

Here is some of the most popular content posted on LCGC International this week.

Canals of Bruges, Belgium | Image Credit: © gqxue - stock.adobe.com

In Bruges: HPLC 2025

Gert Desmet;Deirdre Cabooter;Frédéric Lynen;Sebastiaan Eeltink;Ken Broeckhoven
November 13th 2024
Article

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15-19, 2025, in Bruges, Belgium.

Generate a list of online forums or discussion groups for additional support (Generated with AI) | Image Credit: © ngstock - stock.adobe.com

Recent Trends and Developments in Sample Preparation

Will Wetzel
November 13th 2024
Article

LCGC International spoke with various corporate personalities about trends and developments in sample preparation.

Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos

Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos

Aaron Acevedo
November 12th 2024
Article

LCGC International interviewed FeMS Empowerment Award winner Marta Relvas-Santos on her use of mass spectrometry to identify potential biomarkers and therapies for bladder cancer. She also shared insights on her work with FeMS and advice for fellow scientists.

Molecule of mRNA, 3D illustration (Generated with AI) | Image Credit: © Dr_Microbe - stock.adobe.com

mRNA Characterization, from 5’ Cap to Poly (A): What Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC) Analysis Can Tell You

Roxana Eggleston-Rangel
November 12th 2024
Article

This article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.