Tosoh Bioscience

Biotherapeutics such as monoclonal antibody-derived molecules as well as their impurity profiles are becoming more complex. That challenges the routinely applied analysis of high- and low-molecular weight impurities by size exclusion chromatography and UV-absorbance. Possible limitations of SEC-UV can be overcome by adding advanced detection technologies such as multiangle light scattering (MALS) and mass spectrometry (MS). This podcast will explain the different detection methods and the results they deliver. Special emphasis will be placed on the higher requirements for size exclusion columns and methods preceding advanced detection methods.

Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.

Thursday, April 7, 2022 at 9am EST | 6am PST | 2pm GMT | 3pm CET Thursday, April 7, 2022 at 2pm EST | 11am PST | 7pm GMT | 8pm CET This webinar will present an analytical method to determine several key AAV quality attributes (capsid titer, absolute molecular weight, empty/full ratio, size, and aggregate content) using size-exclusion chromatography (SEC) coupled to multi-angle light scattering (MALS).

To monitor FcR receptor binding, a recombinant FcγRIIIa-ligand has been commercialized into TSKgel FcR-IIIA high-performance affinity chromatography (AFC) columns that separate antibodies based on affinity differences to FcγRIIIa.

Polymers for life-science applications have different attributes from traditional applications such as automotive and packaging. These attributes have their own set of challenges in the characterization requirements of such complex molecules. Multi-angle light scattering (MALS) technology provides a more insightful tool.

Wednesday, November 3, 2021 at 11am EDT | 10am CDT | 3pm GMT | 4pm CET Learn a new and effective method to assess drug potency of mAbs and ADCs. FcR-IIIA affinity chromatography is the latest technique that can profile mAbs and their underlying glycan pattern.

Biomolecules such as proteins, antibodies or viral vectors are becoming increasingly important therapeutics, as evidenced by clinical trials and ultimately drug approvals. But they are also significantly different from small molecules, which affects their HPLC analysis. We explain the differences and what that means for the HPLC conditions and modes used to analyze biomolecules.

This application note demonstrates the scalability from analytical to semi-preparative mode while maintaining similar resolution between the two different capacity columns.

A new approach to assess glycan profiles during cell line screening.

This two-step workflow allows for the rapid screening of N-linked glycans for upstream and downstream mAb production.

This application note demonstrates that FcγRIIIA affinity chromatography allows fast assessment of biologic activity and glycoform pattern of antibodies.

The need for enhanced product quality of biomolecules has led to a higher-level of characterization of different critical quality attributes. Size exclusion chromatography (SEC) has been widely used as a technique to provide size and purity. SEC coupled with multi-angle light scattering (MALS) can determine molecular weight (MW) and size (or radius of gyration, Rg) of complex samples. Dr. Wei Lu, Ph.D. and Philip Hoang from Tosoh Bioscience discuss how SEC-MALS as an analytical method can be optimized and applied to a wide range of applications including oligonucleotides, monoclonal antibodies, and virus-like particles.

*Tuesday, June 1, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT & Wednesday, June 2, 2021 at 12:30pm BST| 1:30pm CEST| 7:30am EDT* John Knox played a pivotal role in the evolution and understanding of both gas and liquid chromatography and is perhaps best known for the development of the Knox Equation, which describes the different contributions to overall band broadening of a solute. *On Demand June 2, 2021*

***Thursday and Friday May 13-14 | May 20-21, 2021*** In the relaxed and unconventional talks in this symposium, highly experienced speakers from industry and academia will impart key learnings from their long experience with chromatography—from heroic failures that have taught them valuable lessons, to moments of inspirational serendipity, and everything in between. CHROMTalks will deliver lifetimes of learning in condensed form, covering topics across implementation, troubleshooting, and method development of analytical techniques. Time spent at CHROMTalks will pay back as soon as you enter your laboratory! ***Available On Demand Until May 21, 2022***

*** Wednesday, May 5, 2021 at 11am EDT | 8am PDT | 4pm BST | 5pm CEST*** A guide to enable users to acquire high quality intact protein mass spectra by inline SEC-ESI-MS will be presented. Gain knowledge on the use and optimization of volatile mass spectrometry-friendly buffers in U/HPLC-MS methods for the analysis of intact mAbs. *On demand available after final airing until May 5, 2022*