What’s Light Got to Do with It? How Light Scattering Relates to Molecular Weight and Size of Biomolecules and Polymers
The need for enhanced product quality of biomolecules has led to a higher-level of characterization of different critical quality attributes. Size exclusion chromatography (SEC) has been widely used as a technique to provide size and purity. SEC coupled with multi-angle light scattering (MALS) can determine molecular weight (MW) and size (or radius of gyration, Rg) of complex samples. Dr. Wei Lu, Ph.D. and Philip Hoang from Tosoh Bioscience discuss how SEC-MALS as an analytical method can be optimized and applied to a wide range of applications including oligonucleotides, monoclonal antibodies, and virus-like particles.
Characterizing Plant Polysaccharides Using Size-Exclusion Chromatography
April 4th 2025With green chemistry becoming more standardized, Leena Pitkänen of Aalto University analyzed how useful size-exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4) could be in characterizing plant polysaccharides.
Investigating the Protective Effects of Frankincense Oil on Wound Healing with GC–MS
April 2nd 2025Frankincense essential oil is known for its anti-inflammatory, antioxidant, and therapeutic properties. A recent study investigated the protective effects of the oil in an excision wound model in rats, focusing on oxidative stress reduction, inflammatory cytokine modulation, and caspase-3 regulation; chemical composition of the oil was analyzed using gas chromatography–mass spectrometry (GC–MS).
