What’s Light Got to Do with It? How Light Scattering Relates to Molecular Weight and Size of Biomolecules and Polymers
The need for enhanced product quality of biomolecules has led to a higher-level of characterization of different critical quality attributes. Size exclusion chromatography (SEC) has been widely used as a technique to provide size and purity. SEC coupled with multi-angle light scattering (MALS) can determine molecular weight (MW) and size (or radius of gyration, Rg) of complex samples. Dr. Wei Lu, Ph.D. and Philip Hoang from Tosoh Bioscience discuss how SEC-MALS as an analytical method can be optimized and applied to a wide range of applications including oligonucleotides, monoclonal antibodies, and virus-like particles.
Polysorbate Quantification and Degradation Analysis via LC and Charged Aerosol Detection
April 9th 2025Scientists from ThermoFisher Scientific published a review article in the Journal of Chromatography A that provided an overview of HPLC analysis using charged aerosol detection can help with polysorbate quantification.
Analyzing Vitamin K1 Levels in Vegetables Eaten by Warfarin Patients Using HPLC UV–vis
April 9th 2025Research conducted by the Universitas Padjadjaran (Sumedang, Indonesia) focused on the measurement of vitamin K1 in various vegetables (specifically lettuce, cabbage, napa cabbage, and spinach) that were ingested by patients using warfarin. High performance liquid chromatography (HPLC) equipped with an ultraviolet detector set at 245 nm was used as the analytical technique.
