Advanced detection (MALS, MS) for size exclusion analysis of proteins - what it offers and what it demands
Biotherapeutics such as monoclonal antibody-derived molecules as well as their impurity profiles are becoming more complex. That challenges the routinely applied analysis of high- and low-molecular weight impurities by size exclusion chromatography and UV-absorbance. Possible limitations of SEC-UV can be overcome by adding advanced detection technologies such as multiangle light scattering (MALS) and mass spectrometry (MS). This podcast will explain the different detection methods and the results they deliver. Special emphasis will be placed on the higher requirements for size exclusion columns and methods preceding advanced detection methods.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
LC-MS/MS Analysis Offers Insights into Intestinal Microbiota and Metabolites in Camels
December 18th 2024A recent study examines the specific traits of the intestinal microbiota and metabolites in Bactrian camels. The results of profiling 36 intestinal mucosal samples from six Bactrian camels using liquid chromatography with tandem mass spectrometry (LC-MS/MS) provides valuable data for understanding the relationship between regional intestinal immunity and the general health and disease of the host.
