Tosoh Bioscience

Combining high-resolution size exclusion chromatography columns with highly sensitive MALS detection characterizes oligonucleotides and structurally-related impurities.

***Live: Tuesday, March 16, 2021 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET*** In this webcast we will provide our top 10 tips for selecting HPLC columns. We will consider everything from analyte-stationary phase interactions, to solid support materials, and how column dimensions can impact separations; and everything in between! *** On demand available after final airing until Mar. 31, 2021.***

AEX using a TSKgel® DNA-NPR column offers a rapid method for discriminating between covalently closed circular, open circular, and linear forms of plasmid DNA.

***Live Dates:Day 1, Wednesday, November 18, 2020 9am–11:30am EST | 2pm – 3:30pm GMT | 3pm-4:30pm CET Day 2, Thursday, November 19, 2020 9am–11:30am EST | 2pm – 3:30pm GMT | 3pm-4:30pm CET ***Biopharmaceutical analysis is a fast-paced and constantly evolving field, and chromatographers play a pivotal role. This two-day virtual event, focusing on cutting-edge trends, has been organized by The Chromatographic Society (“ChromSoc”) in collaboration with LCGC and features talks from top analytical chemists working in biopharmaceutical analysis.***On demand available after final airing until Nov. 19, 2021***

Elution profiles based on mAb glycan structures produced by the TSKgel® FcR-IIIA-NPR™ affinity chromatography column correlate with results from HILIC glycan profiling.  

***Live: Wednesday, October 28, 2020, 11am EDT| 8am PDT| 3pm GMT| 4pm CET*** Discover a new and unique tool to assess the variances in mAb glycoforms and their differences in potency and efficacy using an affinity Fc-Receptor based chromatographic methodology. Data obtained can be correlated to ADCC activity. *** On demand available after final airing until Oct. 28, 2021.***

***Live: Two Day Event! Day One: Thursday, Oct. 22, 2020 10am EDT | 12:30pm EDT | 2pm EDT & Day Two: Friday, Oct. 23, 2020 10am EDT | 2pm EDT *** In this two-day online event, speakers will discuss theory and applications of gas chromatography (GC) and high performance liquid chromatography (HPLC). We have a morning and afternoon session each day, plus a lunchtime tutorial session on 2D-GC on Day 1. There will be live Q&A for the morning sessions and the tutorial. Attendance is free..*** On demand available after final airing until Oct. 23, 2021.***

How UHP‑SEC‑MALS analysis using a UHPLC compatible multi-angle light scattering detector can be applied to determine absolute molecular weight of recombinant proteins.

Online detection of absolute molecular weight of two recombinant protein samples using a 2 μm TSKgel® SEC column directly connected to the LenS3™MALS detector is shown.

Having a good troubleshooting strategy which can be applied to all problems is essential, therefore, using examples of real-life chromatographic problems we will provide you with the knowledge you need to become the expert troubleshooter in your lab. Live: Wednesday, March 18, 2020 at 11am EDT | 8am PDT | 3pm GMT | 4pm CET On demand available after final airing until Mar. 31, 2020 Register free

The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.

The oligonucleotide therapeutics field has seen remarkable progress over the last few years and oligonucleotides are increasingly recognized as potential therapeutic agents for a variety of diseases. Here we describe the ability of ultra-high pressure size exclusion chromatography (SEC) to distinguish N and N-1 oligonucleotide species.

Multidimensional LC/MS is a powerful tool to gain info in the laboratory. Analyzing ADCs with multidimensional LC/MS provides fast data for CQAs.

Join this webcast to hear from leading scientists at Genentech about 2D and 4D LC–MS methods that have enabled more effective and streamlined characterization of critical quality attributes of antibody–drug conjugates (ADCs), in a workflow that is much shorter than traditional approaches. Live: Thursday, December 12, 2019 at 11am EST | 8am PST | 4pm GMT | 5pm CET On demand available after final airing until Dec. 12, 2020 Register free

This note demonstrates the ability of a TSKgel® UP-SW2000 SEC column to discriminate oligonucleotides differing by one base in length.

• Enhanced sensitivity and range are needed to obtain molecular weight and size. • New light scattering detector addresses flaws of traditional approaches.

In this note, the molecular weight averages and molecular weight distributions of two hydroxyethyl starch (HES) samples were successfully demonstrated.

This application note demonstrates that SEC/MS and HIC/UV can be effectively used to characterize the drug-to-antibody ratio profile of antibody drug conjugates.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial mechanism of action (MoA) of anti-tumour therapeutic antibodies and FcγIIIa receptor plays a key role in this process by interacting with the N-glycans of IgG Fc regions. Hence, affinity chromatography on Fc receptor ligands can deliver valuable information about expected ADCC activity and mAb glycoform distribution.

The purpose of this study is to demonstrate the use of mass spectrometry to characterize the elution profile of a typical IgG1 molecule separation on a TSKgel FcR-IIIA-NPR column, and verify the observations that certain glycan structures impart higher activity to the monoclonal antibody, especially as it relates to the presence of terminal galactose sugars.