The Special Requirements of Biomolecules in HPLC Analysis with Andrea Krumm

Podcast

Biomolecules such as proteins, antibodies or viral vectors are becoming increasingly important therapeutics, as evidenced by clinical trials and ultimately drug approvals. But they are also significantly different from small molecules, which affects their HPLC analysis. We explain the differences and what that means for the HPLC conditions and modes used to analyze biomolecules.

The Special Requirements of Biomolecules in HPLC with Andrea Krumm
Current Time 0:00
Duration 0:00
Loaded: 0%
Stream Type LIVE
Remaining Time 0:00
 
1x
    • Chapters
    • descriptions off, selected
    • captions off, selected
      Download Q&A

      Download Q&A

      Download PDF
      Recent Videos
      The Challenges of Characterizing PFAS in the Environment
      The Challenges of Characterizing PFAS in the Environment
      Inside the Laboratory: The Mead Group at UNC Wilmington and the North Carolina PFAS Testing Network
      Inside the Laboratory: The Mead Group at UNC Wilmington and the North Carolina PFAS Testing Network
      Exploring Molecular Dynamics: An Interview with Ezgi Gürler of FeMS
      Exploring Molecular Dynamics: An Interview with Ezgi Gürler of FeMS
      Developing Advanced Fragmentation Schemes: An Interview with Ezgi Gürler of FeMS
      Developing Advanced Fragmentation Schemes: An Interview with Ezgi Gürler of FeMS
      Jacqueline Hamilton, Diane Turner, and Caroline Gauchotte-Lindsay © Images courtesy of interviewees
      Advancing Science Through Diversity: A Conversation with Women in Chromatography, Part 5
      Importance of Collaboration
      Importance of Collaboration
      Evolving Regulatory Landscape
      Evolving Regulatory Landscape
      Analysis of Nitrosamine Intermediates
      Analysis of Nitrosamine Intermediates
      Reference Standards
      Reference Standards
      Addressing Sources, Reformulations, and Upcoming Regulatory Deadlines
      Addressing Sources, Reformulations, and Upcoming Regulatory Deadlines
      Related Content
      Foliage background with a variety of vibrant plant leaves showing a diverse ecosystem and the biodiversity of nature. | Image Credit: © Leigh Prather - stock.adobe.com

      Characterizing Plant Polysaccharides Using Size-Exclusion Chromatography

      Aaron Acevedo
      April 4th 2025
      Article

      With green chemistry becoming more standardized, Leena Pitkänen of Aalto University analyzed how useful size-exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4) could be in characterizing plant polysaccharides.

      Ep. 35: On the Subject of Continuing Education for Spectroscopists

      Ep. 35: On the Subject of Continuing Education for Spectroscopists

      April 1st 2025
      Podcast
      Silvia Radenkovic on Her Research and Passion for Scientific Collaboration

      Silvia Radenkovic on Her Research and Passion for Scientific Collaboration

      Aaron Acevedo
      April 3rd 2025
      Article
      Ep. 34: A Banner Year for Separation Science at Pittcon 2025

      Ep. 34: A Banner Year for Separation Science at Pittcon 2025

      March 14th 2025
      Podcast
      Examining the Relationship Between Vitamin D Levels and Functional Values of Athletes’ Hearts with HPLC–MS/MS

      Examining the Relationship Between Vitamin D Levels and Functional Values of Athletes’ Hearts with HPLC–MS/MS

      John Chasse
      April 3rd 2025
      Article

      Researchers attempted to ascertain the relationship between vitamin D levels in athletes and the structural and functional values of their hearts assessed through electrocardiographic and echocardiographic parameters. Vitamin D levels were measured using high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS).

      Investigating the Protective Effects of Frankincense Oil on Wound Healing with GC–MS

      Investigating the Protective Effects of Frankincense Oil on Wound Healing with GC–MS

      John Chasse
      April 2nd 2025
      Article

      Frankincense essential oil is known for its anti-inflammatory, antioxidant, and therapeutic properties. A recent study investigated the protective effects of the oil in an excision wound model in rats, focusing on oxidative stress reduction, inflammatory cytokine modulation, and caspase-3 regulation; chemical composition of the oil was analyzed using gas chromatography–mass spectrometry (GC–MS).