Application Notes

Many methods have been proposed to determine acylglycerols in biodiesel samples. This analysis is commonly performed by GC/FID (ASTM D6584/EN14105 methods).

In the production of biopharmaceuticals, there may be different analytical requirements for groups performing clone section, formulations and stability, and quality control (QC).

The fully automated combustion ion chromatography (CIC) system presented here combines a highly efficient combustion system with the separation power of ion chromatography (IC).

This paper describes the use of the AB SCIEX TripleTOF 5600 LC–MS-MS system for the screening and identification of unexpected contaminants in lake water, demonstrating the value of high-resolution, accurate-mass data, and retrospective data processing for the identification of nontargeted compounds.

Methacholine chloride is a synthetic analogue of the neurotransmitter acetylcholine used to assess bronchial asthma of subjects in respiratory function labs and epidemiological field studies (1).

The speed and accuracy of software-assisted identification of metabolites can be further improved by combination with rule-based metabolite prediction software.

For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.

Heparin is well-known as an anticoagulant, antithrombotic drug. Chemically, it is a linear polysaccharide that is derived from animal tissues. For some time it has been known that heparin is not a homogeneous substance; rather, it is a heterogeneous mixture of molecules ranging in molar mass from less than 5,000 to more than 30,000 Daltons.

Since 2001 when they became available in North America, monolithic silica HPLC columns have become valuable analytical tools in the R&D and methods development laboratories.

The authors look at comprehensive two-dimensional gas chromatography as a choice for the quantitative speciation of trace alkyl phosphates in petroleum samples.

The EcoSEC GPC System and a set of TSK-GEL mixed-bed columns, both available from Tosoh Bioscience, were used successfully for obtaining high quality MWD data of a series of block poly-β-alkylalanoids with HFIP as the mobile phase in under 15 min.

The RegisPack® chiral stationary phase allows for the enantiomeric separations of a wide variety of racemate classes.

The analysis of melamine in milk and other food products became urgent when first the pet food, and later the milk scandal were discovered in the U.S. and China.

One of the most important attributes of flexible fused silica capillary tubing is its outstanding strength. When made properly with a protective abrasion resistant coating and handled appropriately this material has proven to maintain its strength remarkably well.

Analyzing basic pharmaceuticals and peptides can be challenging with conventional ODS columns.

The U.S. EPA Office of Groundwater and Drinking Water released Method 524.3, "Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry, Version 1.0," in the Federal Register on August 3rd, 2009 (1, 2).

In ion chromatography, the presence of a large amount of matrix ions makes quantification of the target ions difficult.

Biomolecules, such as proteins and peptides, represent a growing number of pharmaceutical drug entities.

Ultra fast separations of acetaminophen, caffeine, propofol, and parabens are achieved in 30 s using the Accelaâ„¢ UHPLC system with the 80 Hz PDA detector.

The extraction and analysis of semi-volatile organic (SVOC's) compounds can be problematic due to the wide range of analyte types and polarities.

Glyphosate (GP) is a non-selective, postemergence herbicide widely used for weed and vegetative control.

Pyrolysis GC?MS is a valuable tool for the material scientist. It is used principally for qualitative identification of polymers and their breakdown products. This application note displays how this technique can also be used for quantitative determination of polymer compositions by comparing peak area ratios of monomers and/or primary pyrolysis products.

Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.

Thermogravimetric analysis (TGA) measures the change in the weight of a sample as a function of temperature.

Capillary PLOT columns can be a simpler alternative to packed column setups for propylene analysis. Here we demonstrate a single column setup that provides fast baseline resolution of propylene impurities.

The determination of PCBs, as well as other environmental contaminants, in fish tissue requires extensive sample cleanup prior to analysis by gas chromatography with an electron capture detector (GC-ECD).

Spice and fragrance essential oil raw materials frankincense (Boswellia carterii) from India and Somalia, sandalwood (Santalum album) from India and Indonesia, and corn mint (Mentha haplocalyx) from the United States were analyzed for on-site analytical profiling and confirmation of quality and purity of therapeutic grade essential oils from raw materials.

ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8–C40 aliphatics, C10–C22 aromatics).

The Agilent 1290 Infinity LC has significant capabilities for a wide range of HPLC and UHPLC applications.

The stevia plant and its extracts have long been used as sweeteners in Asia and Latin America. Two steviol glycosides present in plant tissue, stevioside, and rebaudioside A, are largely responsible for the sweet flavor (1).