Multidimensional LC

Peter Schoenmakers of the University of Amsterdam presented a talk at HPLC 2023 on multidimensional liquid chromatography (LC).

Scientists have developed a breakthrough MDLC–MS method for in-depth characterization of charge variants in therapeutic antibodies. The innovative approach enables the detection and evaluation of rare charge variants, facilitating improved safety and efficacy in antibody development.

Organic acids, flavonoids, iridoids, and saponins were among the compounds discovered, with significant α-glucosidase inhibitory behavior in vitro that was inconsistent with substance content level.

Researchers have developed an innovative strategy for compound annotation in offline 2D-LC–MS analysis. The approach combines hand-in-hand alignment with targeted molecular networking (TMN) and was successfully applied to analyze the chemical profile of Yupingfeng (YPF), a traditional Chinese medicine prescription, resulting in the identification of 497 compounds and demonstrating its efficiency and scalability in complex sample analysis.

This study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.

The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.

An analytical team has successfully developed a novel strategy for the separation of sanshools from Zanthoxylum bungeanum oleoresin using countercurrent chromatography, providing valuable insights for the separation of compounds with similar chemical properties.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

A new study introduces a comprehensive two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC–MS/MS) method for the analysis of 112 pesticides in corn-based products. The method exhibits high precision, lower limit of quantification values, and successful detection of trace levels of pesticides in real samples, offering promise for the analysis of complex matrices.

The evolution of biomolecule analysis is discussed in this article.

At Pittcon 2023, LCGC spoke with Jim Grinias of Rowan University. This segment is the full-length version of the interview that was conducted at the conference.

A new article discusses the benefits and drawbacks of RPLC and RPLC×RPLC chromatography techniques. It provides recommendations for selecting the best approach for analyzing small pharmaceuticals and peptides.

A recent study shows how 2D-LC–MS can provide expanded analytical coverage of cosmetics-prohibited substances.

On a fundamental level, equilibrium and diffusion define chemical separations. Practical applications of partition coefficients to chromatographic sample preparation illustrate this point.

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.

This review article summarizes the results obtained from the combined efforts of a joint academic and industrial initiative to solve the real-life challenge of determining low levels of peptide-related impurities in the presence of the related biologically-active peptide at a high concentration.

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

This article explores potential pitfalls associated with 1D‑LC and how 2D‑LC can overcome these obstacles.

Having a mixed-mode column that can provide both achiral and chiral resolution in one-dimension (1D) would significantly improve impurity profile understanding and reduce testing workload.

In this article, I share my perspective on the trends in 2D-LC, and the developments we are likely to see in the field in the near future.

As two-dimensional liquid chromatography (2D-LC) becomes more widely used, system suitability tests (SSTs) become even more important.

This month we interview Alexandre Goyon, Senior Scientist in the Small Molecule Pharmaceutical Sciences Organization of Genentech, about his work focused on the online digestion and analysis of RNA molecules using immobilized RNase cartridges attached to a mDLC–MS system, and why the accurate sequencing of sgRNA is important.

Multidimensional liquid chromatography (MDLC) methods have revolutionized the characterization of complex drug modalities like antibodies–drug conjugates, antisense oligonucleotides, and small interfering RNA therapeutics.

Two-dimensional LC, with advanced modulation techniques, can advance the analysis of advanced polymeric materials, assisting in characterizing copolymer composition heterogeneity and identifying ingredients in complex products.

James P. Grinias is an Associate Professor in the Department of Chemistry & Biochemistry at Rowan University in Glassboro, New Jersey, and the winner of the 2022 Emerging Leader in Chromatography Award, which is presented by LCGC magazine.

Barry L. Karger and James P. Grinias are the winners of the 15th annual LCGC Lifetime Achievement and Emerging Leader in Chromatography Awards, respectively, for 2022. Here, we review their achievements.

We review different approaches and coupling strategies for analyzing monoclonal antibody aggregates with 2D-LC.

In 2D-LC, properties of the mobile phase used in first step can negatively affect the second step. We explain why this problem happens and how to avoid it.

Filip Cuyckens from Janssen R&D in Belgium spoke to LCGC Europe about recent innovative approaches he and his team developed to support drug metabolism and pharmacokinetic studies, and the inventive role that two-dimensional liquid chromatography (2D-LC) plays in his laboratory to boost sensitivity, solve recovery issues, and increase overall efficiency.

This article will discuss the benefits of 2D-LC and multiple application areas in (bio)pharmaceutical analysis, and will highlight the challenges and future outlook.