Liquid Chromatography (LC/HPLC)

Examining the role that LC–MS/MS plays in metabolomics, and metabolomics’ growing importance in the field of disease biology.

This article details a recent experiment that demonstrates how nonspecific interactions can be prevented by using metal-free or low adsorption flow paths.

How to modernize an older pharmaceutical monograph from an HPLC column to an UHPLC column, along with calculated time and solvent savings.

Researchers have utilized computational fluid dynamics (CFD) simulations to optimize the design of membrane chromatography devices, enhancing fluid flow uniformity and separation efficiency.

A separation science team has developed a green and cost-effective method, called feather fiber-supported liquid extraction (FF-SLE), for determining synthetic antioxidants in edible oils.

Researchers have introduced a novel primary method utilizing gas chromatography–isotope dilution infrared spectrometry (GC–IDIR) for accurate peptide purity analysis.

Researchers have developed a robust method utilizing high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) to accurately quantify amino acids in plasma, making it a valuable tool for clinical diagnostics and research in amino acid metabolism.

Enhancements in chromatographic performance were reviewed as were experimental concerns, both pointing toward using specific solvents to influence more sustainable industrial development.

Pop quiz – can you define resolution? Would you be surprised at the number of correct answers? Which one is the best for chromatographers? In this LCGC Blog, we look at the various ways resolution can be interpreted scientifically.

Levoglucosan, which is emitted from the combustion of cellulose and hemicelluloses, can be transported over a long range of time to ice caps, where it may indicate fire activity both regionally and globally.

Researchers have discovered that phospholipids form doubly charged lipid-metal ion complexes during electrospray ionization (ESI), providing valuable insights into the analysis of phospholipid structures.

Researchers have developed an ultrahigh-pressure liquid chromatography (UHPLC)—tandem mass spectrometry (MS/MS) method for the simultaneous determination of 10 aminoglycoside antibiotics in aquatic products.

Researchers have introduced MCnebula, a new framework for untargeted LC–MS/MS data analysis. MCnebula focuses on critical chemical classes and utilizes visualization techniques to streamline the analysis process, enabling efficient pathway analysis, biomarker discovery, and classification of unknown compounds beyond the limits of spectral libraries.

A new method has been developed for detecting and quantifying polycyclic aromatic hydrocarbons (PAHs) in intermediates used in plastics production. The method, based on liquid chromatography column switching, allows for the analysis of 18 PAHs and addresses the lack of regulatory oversight for PAH concentrations in plastic intermediates.

The team of researchers found that components with the same retention time can have different migration patterns, and discussed the different scenarios for decreasing column pressure resistance.

Researchers have developed a novel algorithm that combines multivariate curve resolution-alternating least-square (MCR-ALS) and Bayesian inference techniques to estimate confidence intervals for the quantitation of coeluted peaks in liquid chromatography-photodiode array detection.

In a lecture at HPLC 2023, Valérie Pichon of ESPCI Paris and Sorbonne University said these advances address limitations of more traditional extraction techniques.

In a presentation at HPLC 2023, Kevin Pagel of Freie Universität in Berlin, Germany described methods he has found most effective for better understanding the roles of glycans and glycoconjugates in biological processes.

At HPLC 2023, Paola Dugo held a lecture on how combining two-dimensional liquid chromatography (LC×LC) with mass spectrometry (MS) can help scientists analyze food samples.

At HPLC 2023, Erin Baker held a lecture on how using liquid chromatography and mass spectrometry together can help scientists further understand diseases that can arise from chemical exposure.

In his plenary lecture at HPLC 2023, Michal Holčapek presented his team's findings on how chromatography can help scientists record the human lipodome and better our understanding of diseases.

Gunda Köllensperger spoke at HPLC 2023 about how using U13C-isotopically labelled biomass extracts can change how scientists approach metabolomics.

In this HPLC 2023 keynote lecture, Mario Thevis discusses how chromatographic–mass spectrometric approaches have led to important advancements in sports drug testing programmes.

At HPLC 2023, Hyukju Kwon hosted a lecture on the roles LC–MS and artificial intelligence can have in autonomous material discovery.

Peter Schoenmakers of the University of Amsterdam presented a talk at HPLC 2023 on multidimensional liquid chromatography (LC).

Award presentations and plenary lectures were some of the attractions as the annual conference got underway on Sunday.

At HPLC 2023, Oliver Fiehn of the University of California explained results gathered from a device that can be used during daily digestion.

In a session on omics at HPLC 2023, Robert T. Kennedy of the University of Michigan revealed how scientists are taking advantage of new developments and cutting down on analysis time.

At HPLC 2023, Kelly Zhang gave a lecture on how machine learning can help further our understanding of the characterization of lipid nanoparticles.

At HPLC 2023, Matthias Pursch presented his findings on using UHPLC and SEC to monitor small molecule and polymer reactions in real time.