Yuki Suzuki earned her Ph.D. in neurogenetics from Tokyo Metropolitan University in 2022. She currently works as an application chemist at the Solution Center of Excellence at Shimadzu Corporation in Japan, where she focuses on liquid chromatography–mass spectrometry (LC–MS). Dr. Suzuki has developed a wide range of applications across various fields, including life sciences, food safety, and environmental analysis.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.