December 20th 2024
This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Problems and Solutions of Landmine and Improvised Explosive Devices
February 25th 2007As most of us traveled by air to Chicago this weekend, we all passed through airport security and the various drills like taking off our shoes, removing metal objects and taking the all-important laptop out of its case.
Liquid Chromatography at the Extreme: Ultra-Pressure, High Temperature, and Ultra-Fast
February 25th 2007Pittcon 2007 opened with a bang here in Chicago, albeit a snowy bang. All the liquid falling outside, whether in rain, snow, or ice form, was nothing compared to the liquid chromatography sessions.
Separation Instrumentation Demand
February 20th 2007Separation instrument techniques are among the most widely used technologies in the analytical instrumentation market. It spans the entire industrial and regional marketplace. The lab separations market includes chromatographic techniques like analytical and preparative HPLC, GC, IC, TLC, flash, and low pressure LC.
Technology Forum: Pittcon 2007
February 20th 2007Each month in our Technology Forum we will feature a discussion between industry experts on various trends and issues in the chromatography field. This month's Technology Forum looks at the topic of Pittcon 2007 and the trends and issues surrounding it. Joining us for this discussion are Tom Ricci with Ricci Communications and LCGC Columnists John Dolan and John Hinshaw.
High Performance Liquid Chromatography
February 1st 2007HPLC is among the most dynamic markets in the laboratory analytical and life science instrument industry. The market can be segmented into six different categories, which include conventional HPLC, capillary/nano LC, fast LC, preparative HPLC, amino acid analyzers, and gel permeation chromatography systems. Conventional HPLC systems, which account for the majority of the market, are standard HPLC instruments with typical flow rates between 1–10 mL/min. However, other HPLC systems designed for specific applications and that remedy some of the shortcomings of conventional LC systems, are becoming more significant in the market.
Facilitated Column Selection in Reversed-Phase Liquid Chromatography for Pharmaceutical Separations
This article gives an overview of the performance of a previously developed system for the ranking of C18 reversed-phase columns applied to different pharmaceutical analyses. The separation of eight different drug substances from their respective impurities was studied. The chromatographic procedure for acetylsalicylic acid, clindamycin hydrochloride, buflomedil hydrochloride, chloramphenicol sodium succinate, phenoxymethylpenicillin and nimesulide was performed according to the corresponding European Pharmacopoeia monograph. The separations of dihydrostreptomycin sulphate and vancomycin were performed according to literature. It was found that that the column ranking system is a helpful tool in the selection of suitable columns in these analyses.
Ask the Editor: Technical Editor Steve Brown Answers Your Questions!
January 28th 2007Beginning in February, Steve Brown, LCGC technical editor of 18 years, will answer your technical questions. Each month, one question will be selected to appear in this space, so we welcome your submissions. Please send all questions to the attention of "Ask the Editor" at lcgcedit@lcgcmag.com. We look forward to hearing from you.
Regis Technologies Expands Its GMP-Approved Chiral Separations Services with SFC
January 15th 2007Regis Technologies, Inc. partners with pharmaceutical, biotechnology, and other companies to help expedite drugs to market by providing synthesis and separations services. Regis Technologies announced today it has added Supercritical Fluid Chromatography (SFC) to its separations services and will offer GMP separations April 1, 2007.
Practical Comparison of Different Approaches to Speed Up LC Separations
January 1st 2007This article describes and compares a number of approaches to increase the speed of liquid chromatographic separations. On a standard LC column, a gain of a factor two in the run time (from 10 to 5 minutes) was achieved by increasing the flow-rate two-fold. On a monolithic column, a column operated at high temperature (120 ?C) and a short column, flow-rates could be increased typically five-fold, resulting in run times in the order of 2 minutes. This was accompanied by a sometimes considerable loss in separation efficiency. A combination of a very short run time and unaffected separation efficiency was realized on a UPLC system, designed for use at higher pressure. By working at approximately 800 bar, the analytes could be well separated within 30 seconds.
Market Profile: Water Analysis
January 1st 2007The environmental market peaked about fifteen years ago when the United States government passed a large body of new environmental laws and strengthened existing ones, dramatically increasing the number of mandated tests. As a result, the analytical instruments market received a boost in new instrument sales. While growth from the environmental market has slowed considerably since, the total market is still quite significant.
Innovations in Food Analysis — Analysis of Peptides, Additives and Flavours
December 2nd 2006The analysis of peptides and additives using a new HPLC innovative column technology, Pathfinder, was shown. For the analysis of flavours in lemon juice GC–MS with a new MS library, FFNSC, with linear retention indices was used.
Determination of Soft Drink Ingredients
December 2nd 2006Soft drink formulas often include preservatives, artificial sweeteners, flavours or caffeine in their list of ingredients. Using the Acclaim OA column, as many as eight common additives may be determined in a single run. Many of these additives are hydrophilic organic acids for which this column was designed. Notably, benzoate and sorbate, which do not resolve on C18 columns at low pH, are fully separated.
Optimizing Column Lifetime of TSK-GEL Size Exclusion Columns
December 2nd 2006Column lifetime is a more and more important issue when developing an analytical method for HPLC. Besides sample treatment, column cleaning and storage, operational parameters of the analytical method will have an influence on column lifetime. This question may not always be addressed early enough in the methods development process.
Data-Handling Software for a GLP Environment: Development and Validation Requirements
December 1st 2006In a good laboratory practice (GLP) environment, data-handling software cannot be used until it is validated. This even applies to the most simple program that performs calculations or stores data. A detailed documentation of the set-up and the performance of the software - called software validation - is required. The development and validation requirements are described in this article and illustrated with a software for robustness testing (SRT), which guides the user step-by-step through the experimental set-up and interpretation of robustness tests. This software was developed in an Excel (Windows XP) environment and is used as part of method validation in laboratories that require compliance with GLP and 21 CFR Part 11. The software was subjected to software validation regulations and is compliant with electronic records and signature rules (21 CFR Part 11) as it creates, delivers and stores electronic data. The validation tests are based on the computerized system validation (CSV) -..