Partial-Filling Techniques for Affinity Capillary Electrophoresis to Probe Receptor-Ligand Interactions
Analysis of Combinatorial Natural Products by HPLC and CE
The authors used HPLC and CE to monitor changes in combinatorial genetics mediated secondary metabolite generation.
An Improved Analytical Temperature-Rising Elution Fractionation System for Automated Analysis of Polyethylenes
The authors describe a system for automated analytical temperature-rising elution fractionation that uses the built-in differential refractometer of a commercial gel-permeation chromatography instrument and a stop-flow method for sample crystallization.
In-Tube Solid-Phase Microextraction and On-Line Coupling with High-Resolution GC
The guest authors describe an in-tube SPME system coupled with capillary GC for the trace analysis of organic contaminants in water.
The Evolution of the Application of Gas Chromatography
Leslie Ettre discusses four major application fields in which GC changed the way chemists carry out analyses.
Broad Peaks
John Dolan discusses system-related causes of peak broadening in isocratic reversed-phase LC.
Gas Buying 101 — Eliminating the Hassles Associated with Obtaining and Using Gases
Guest author Steve Scheuring suggests ways to choose and use gases in laboratories.
Validation of Impurity Methods, Part II
Second column in a series that reviews impurity method validation guidelines.
2003 Index of Authors and Subjects
2003 index of authors and subjects.
Understanding Split Peaks
This month's column looks at several possible causes of peak splitting and presents some guidelines for isolating its source.
Product Showcase
Products and literature for separation science.
Are You Getting the Most Out of Your HPLC Column?
Majors discusses low-dispersion columns, describes how to modify HPLC systems to work with the small-volume columns, and provides guidance about obtaining the optimum capabilities from these columns.
Representation of Chemical Structures from Partial to Final Structure — Accelerating Mass Spectrometric Interpretation
Advanced Chemistry Development, Inc. For more information, visit the company's website at www.acdlabs.com.
On-line Coupled LC?GC: Theory and Applications
In this article the authors describe the benefits of on-line coupled LC?GC using a series of applications. The pros include shorter analysis times, better reproducibility and improved detection limits.
The Application of Multiplexed Microemulsion Electrokinetic Chromatography for the Rapid Determination of log POW Values for Neutral and Basic Compounds
This article illustrates the use of multiplexed microemulsion electrokinetic chromatography with UV detection to develop a rapid approach for obtaining log POW values of neutral and basic compounds.
Identifying Compounds Despite Chromatography Limitations: Organophosphates in Treated Sewage
The authors descrive the use of GC–MS to isolate and identify potentially toxic compounds in concentrated extracts of sewage treatment plant effluents.
Solid-Phase Microextraction
John Hinshaw explores how to perform solid-phase microextraction and discusses the advantages provided by the technique.
Tissue Sample Preparation — Not the Same Old Grind
The guest authors provide an overview of tissue sample preparation by discussing and comparing conventional extraction technologies an novel approaches.
Extracolumn Effects
The authors show how several factors can contribute to a chromatogram's appearance.
Appendix for "Separation of Toxic Coplanar PCB Congeners . . ."
Source Design and the Utility of Multimode Ionization
This new column looks at the current importance of electrospray ionization and emerging approaches for samples that require ionization by other means, including a new technology, multimode ionization.
Separation of Toxic Coplanar PCB Congeners from Other Congeners in Marine Sediments and Marine Fish Tissues Using Florisil and GC–ECD
This article describes a sample extraction and cleanup procedure for removing toxic PCB congeners from other congeners in polluted marine and estuarine sediment and fish. The resulting extracts are analyzed by GC–ECD.
How Much Is Enough?
Dolan presents two situations in which experimental evidence is used to determine the best operating conditions.
Gas Chromatography at the 26th International Symposium on Capillary Chromatography and Electrophoresis
Hinshaw provides an overview of scientific presentations and technology developments from the 26th International Symposium on Capillary Chromatography and Electrophoresis.
Highlights of HPLC 2003
Majors discusses highlights from HPLC 2003, held June 2003 in Nice, France.
The Beginnings of Gas Chromatography and Early Symposia Held in the United States
This column discusses the beginnings of GC in the United States and four early symposia.
Honoring Readers
Dolan responds to reader comments and questions in celebration of the 20th anniversary of "LC Troubleshooting."
Why Do Peaks Tail?
John Dolan discusses the causes of peak tailing, how to measure it, preventive measures, and why newer column types are less prone to tailing problems.
Validation of Impurity Methods, Part I
This column is the first installment in a two-part series reviewing ICH and FDA impurity method validation guidelines.
Analysis of Various Cyanobacterial Toxins by LC–MS
This article focuses on the initial development of an LC–MS method to screen for cyanotoxins at low parts-per-billion levels.