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The development of a method for the simultaneous determination of glycine, triglycine and fructose using UV/vis and evaporative light-scattering is presented. The study formed part of a research project dealing with the recovery of functional peptides from aqueous streams on an industrial scale using absorption or related technologies.

Liquid chromatography is a crucial process used in making a quickly expanding range of biotech products such as human insulin and cancer-fighting drugs. Located in one of the newer buildings at Pease International Tradeport (Portsmouth, New Hampshire), Euroflow represents a new breed of biotech-related companies that have emerged recently throughout the region.

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The second part in this series focuses on the variables in large data tables, looking at the vocabulary used by PCA experts, and explaining some of the background to the method. It further provides practical examples of how these variables operate in practice.

News All the news from January 2005 Opinion Our columnist, Zosimus, sparks some interesting debate on chromatography issues. Market trends & analysis Glenn Cudiamat provides an insight into the status of the current LC-MS market. Country focus From new appointments to new launches, this French focus will keep you a la page with what's happening in France. Q&A Geof Wyatt of Wyatt Technology provides an interesting interview on light-scattering matters.

In this article, the author describes the development of a simple enzymatic reactor, which also functions as a microdevice that combines both preconcentration and enzymatic digestion in the nano-electrospray emitter, and its interfacing with a mass spectrometer.

In last month's installment of "GC Connections," John Hinshaw discussed how peak retention times depend upon relationships between pressure, flow rate, oven temperature, column dimensions, and stationary phase. This concluding installment of a two-part series discusses the effects that column variability has on isothermal capillary gas chromatography and explores instrument calibration with the goal of maximizing instrument-to-instrument similarity of retention times.

The effects of temperature on HPLC are explored, along with a critical analysis of the effects of temperature change on both peak shape and efficiency.

In this article, the authors examine the concentration-dependent influence of Na+- and K+ ions on mass spectra of peptides, with human gastrin as a model peptide using LC/ESI–MS as the selected ionization technique.

The authors provide suggestions for the sample pretreatment and analysis of aerosol particles including the use of on-line SFE–LC–GC–MS, GCXGC and LC–MS. Although each technique has its merits, the novel multidimensional systems and those integrating sample pretreatment with the final analysis proved to be valuable tools in aerosol analysis.

This article discusses the development of gel permeation chromatography or size exclusion chromatography as a technique for analysing the molecular weight distributions of polymers. It further highlights the development of columns and hardware for high-resolution characterization of polymers.

The authors look at two types of method modification from a scientific and regulatory standpoint: a change of column and a change in the operating system. Both are difficult to avoid if a method is used for several years.

While some variation in retention is normal, larger changes from run to run are generally indicative of a problem. Here, the auther discusses how the retention time of a peak in an LC separation can be a useful diagnostic tool to identify problems with a separation.