Separation of Ionic Analytes via Supercritical Fluid Chromatography
September 1st 2009Supercritical fluid chromatography (SFC) has been practiced for approximately 50 years. SFC on packed columns for both qualitative and quantitative purposes underwent a renaissance in interest at the beginning of the 1990s when limitations of capillary SFC became obvious and important progress in composition gradient techniques for mixed mobile phases was achieved. Even with these instrumental improvements, wide acceptance of the technology was not forthcoming because the perception was that highly polar analytes were not soluble in carbon dioxide and thus were not separable. It is now apparent that the use of additives dramatically extends the range of solute polarity amenable to SFC.
A Novel Sample Preparation Approach to Increase the Throughput of Pesticide Analysis by LC–MS-MS
September 1st 2009The high-throughput analysis of pesticides in raw agricultural commodities has been hindered by the slow and laborious sample preparation stages. Using traditional clean-up procedures, as many as 20 steps are required to get the sample in a convenient form for analysis, which typically limits sample throughput to eight samples per day. This study evaluates a new approach using a novel laboratory mill and pulverizer to extract various pesticide residues from different kinds of plant materials and to identify and quantitate the active ingredients by liquid chromatography–tandem mass spectrometry (LC–MS-MS). It shows that by automating the procedure, the number of clean-up steps can be reduced significantly, which increases sample throughput over traditional approaches.
Thermo Fisher Scientific Conducts Radiation Monitoring Course Near Chernobyl
August 25th 2009Thermo Fisher Scientific (Franklin, Massachusetts) announced that its Radiation Measurement and Security Instruments (RMSI) business has completed its 12th annual radiation monitoring techniques training course outside of Kiev, Ukraine, and within the vicinity of the Chernobyl nuclear power plant.
prominence TOX.I.S. (HPLC/PDA for identification of drugs in human urine)
August 21st 2009Toxicological analysis of body fluids is an important task whenever acute intoxications, abuse of drugs or consumption of illicit drugs needs to be identified. Such analyses require a universal sample pretreatment and identification method and should be automated for speed and simplicity.
The Column: August 2009 Issue 2
August 20th 2009Improving Chromatographic Performance by Using Freshly Delivered Ultrapure Water in the Mobile Phase Maricar Tarun, Coralie Monferran, Cecilia Deveaux and Stephane Mabic A More Efficient Extraction of Melamine from Various Matrices using Resin-based Mixed-mode Cation Exchange SPE and Analysis with LC-MS-MS Lee Williams, Joanna Caulfield and Elena Gairloch News Awards for waste reduction and capillary chromatography, carotenoids for eye care and the buyout of Varian by Agilent are the stories in this issue. Market Trends and Analysis Market Profile: GC-MS Glenn Cudiamat Tips & Tricks: GPC/SEC Temperature Effects in GPC/SEC Thorsten Hofe and Daniela Held Events
Pittcon 2010 Announces Plenary Lecturer
August 20th 2009Pittcon (Pittsburgh, Pennsylvania) announced that Alan G. Marshall, Kasha Professor of Chemistry and Biochemistry at Florida State University (Tallahassee, Florida) and director of the Ion Cyclotron Resonance Program at the National High Magnetic Field Laboratory will be the plenary lecturer during the opening session on March 1 at Pittcon 2010.
Light Scattering Analysis of BSA with ProSEC 300S Columns
August 14th 2009Albumins are globular proteins easily soluble in water and 50% saturated ammonium sulphate solutions. They are found in animal tissue, blood plasma, bacteria and plants, and include many important proteins with key functions in the body.