European leg of scientific solutions tour begins
October 5th 2009The autumn leg of PerkinElmer?s 2009 European ?For the Better? Tour will soon get underway. At this series of seminars, scientists, industry leaders and the company?s specialists will share their applications expertise and demonstrate how the company?s solutions can be used in sectors as diverse as environmental analysis, food safety, polymers and chemicals.
Fast Analysis of Charge Variants of Monoclonal Antibodies by Ion Exchange Chromatography
October 2nd 2009Development and production of biopharmaceuticals is a growing segment of the pharmaceutical industry. The development of vaccines is only one - but as a result of the current H1N1 flu hysteria it is the most striking - segment of this emerging field. Recombinant proteins, ranging from insulin products to therapeutic antibodies, represent another important group of biopharmaceuticals. With the introduction of the first so-called biosimilars in Europe the demand for highly efficient analysis methods is further increasing. Newly developed stationary phases for the typical modes of biochromatography of native proteins such as SEC or IEC open up new opportunities for increasing throughput in the bioanalytical lab.
Rapid Analysis of Corn Stover Acid Hydrolysate for Estimation of Total Monosaccharide Content
October 2nd 2009Corn stover is the above-ground portion of the plant minus the kernels and it accounts for a large percentage of the global supply of lignocelluosic biomass available as feedstock for fermentation systems used for biofuel production.
High Temperature GPC Analysis of Polyolefins with Infrared Detection
October 2nd 2009The analysis of the molar mass distribution of polyethylene and polypropylene resins by GPC/SEC has always been considered a demanding task because of the requirement of high temperature operation for dissolution and complex hardware design, which often results in high maintenance cost, in particular related to the autosampler/injector and detector units, and in other problematic and consuming tasks such as solvent handling added to column fragility, sample degradation or detector sensitivity–stability.
Sensitive Detection of IgG Aggregation using On-line Fluorescent Dye Detection in HP-SEC and AF4
October 2nd 2009To guide the development of aggregate free protein formulations, there is an urgent need for complementary methods that allow a sensitive detection, characterization and quantification of different types of protein aggregates.
Light Scattering for the Masses Protein–Protein Interactions
October 2nd 2009Over-expression of recombinant proteins is commonly used for the production of protein reagents in industry and academia. Problems often occur relating to the stress put on the cells to deal with this huge increase in synthesis. Cellular proteins that are part of the protein synthesis machinery are often up-regulated under such conditions. Large quantities of the recombinant protein can be bound to these cellular proteins, making purification difficult.
High Resolution Peptide Mapping of Ig-G Using Kinetex C18
October 2nd 2009A critical part of research and quality control analysis of proteins involves verifying the primary and secondary structure of a protein. Peptide mapping is the main technique used to determine the structure of a protein as well as identify any post-translational modifications.
Separation of Tryptophan Oxidized Peptides from Their Native Forms
October 2nd 2009Oxidation of amino acid residues can alter a protein's biological activity, half-life and immunogenicity. Peptide maps can be used to detect oxidized peptide fragments. Peptides are commonly separated by RP-HPLC; however, hydrophobic interaction chromatography (HIC) is an alternative technique offering different selectivity.
Trace-Level Aliphatic Amines in Cationic Drugs
October 2nd 2009The analytical challenge treated in the present work consists in determining sub-ppb concentrations of low-molecular-weight amines in the presence of strongly retained cationic drugs by using ion chromatography (IC) with upstream in-line coupled-column matrix elimination (CCME).
Comparison of Ion Trap and Q-TOF Mass Spectrometers for Protein Identification
October 2nd 2009This study uses the challenging analysis of a complex, multi-organ peptide digest of Caenorhabditis elegans (C. elegans) to compare the performance of a novel linear ion trap mass spectrometer and a quadrupole time-of-flight (Q-TOF) mass spectrometer.
Fast Separation of Seven Biocides Using an Agilent ZORBAX RRHD Eclipse Plus C18 1.8 µm Column
October 2nd 2009The flexibility to use short sub-2 micron columns on UHPLC instruments at high flow-rates permits fast analysis with high resolution of complex samples. An Agilent ZORBAX Rapid Resolution High Definition (RRHD) Eclipse Plus C18 1.8 µm column separated seven different biocides in less than 1 minute with high resolution. A flow-rate of 1.7 mL/min was used on 2.1 × 50 mm column to achieve the rapid separation. The Agilent 1290 Infinity LC system was used as the column pressure just exceeded 1000 bar at this high flow-rate. A sample of hand sanitizer was found to contain 2-phenoxyethanol and methylparaben using the method.
The Third Annual LCGC Pittcon Awards: Establishing a Tradition
October 1st 2009They say it takes several years for an awards program to really get off the ground and start amassing a history, establishing a place for itself in the industry. If this is true, then I believe we can safely say that this year, the LCGC Pittcon Awards have officially arrived. With past Lifetime Achievement Award winners such as Walt Jennings and Harold McNair, and bright young stars such as Gert Desmet and Kevin Schug claiming Emerging Leader honors over the past couple of years, there is a great deal of suspense building over who will win the 2010 awards, set to be presented at Pittcon 2010 in Orlando, Florida.