Advanced Profiling Method based on MELDI–MS For High-Throughput Pattern Analysis in Proteomics
October 1st 2009A new proteomic profiling method based on material-enhanced laser desorption/ionization (MELDI) has been developed to identify candidate biomarkers that are selected in MELDI mass profiles. The basic principle of applying MELDI is to trace out the low-concentration species generated as a result of disease, which can then be used as diagnostic markers after their authentic validation. The first step of the MELDI approach is applied to reduce the complexity of proteomic samples by specific binding of serum proteins onto chemically modified MELDI beads, which are then directly analysed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). The resulting mass profiles provide the basis for differentiating diseased samples from control samples. The use of liquid handling robots for sample preparation for high-throughput applications leads to higher reproducibility, which is crucial to succesfully identify disease markers. The ongoing development of MELDI for the..
The Column: September 2009 Issue 2
September 24th 2009Advancements to Take the Pain Out of Chromatographic Peak Integration Shaun Quinn Reducing Time and Cost with Multi-vendor Chromatographic System Control Mark Harnois, Chris Stumpf and Steven F. Eaton Meeting Review: SFC 2009 - 3rd International Conference on Packed Column Supercritical Fluid Chromatography Larry T. Taylor News Market Trends and Analysis Market Profile: Worldwide Ion Chromatography Demand Glenn Cudiamat Events
GC-MS Used to Determine Effects of High Fat Diet During Pregnancy
September 24th 2009Researchers from the University of Utah (Salt Lake City, Utah) and the Oregon National Primate Research Center (Beaverton, Oregon) used gas chromatography-mass spectrometry based metabolomics to analyze blood from mothers and offspring of animals fed a high fat diet.
Thermo Fisher Scientific Wins Food Safety Award for Melamine Detection Program in China
September 22nd 2009Thermo Fisher Scientific (Waltham, Massachusetts) announced that it has received a Ringer Technology Innovation Award for helping Chinese laboratories develop melamine detection methods following the discovery of tainted milk and infant formula in China.