Stephanie N. Harden

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Stephanie N. Harden is the manager of Waters’ global small molecule pharmaceutical market segment, with 20+ years of experience in product and segment marketing. Stephanie has a passion for enabling world-class pharmaceutical development and has been instrumental in helping customers to transform the way their organizations operate. She is a highly cited author with a Ph.D. in chemistry from the University of Bristol, UK.

Articles

Advantages of Hybrid Surface Technology to Improve Chromatographic Analysis of Food, Beverages, and Dietary Supplements

Stephanie N. Harden;Jinchuan Yang;Paul Rainville;Chantel Lee

October 11th 2024

The article discusses the application of a hybrid organic/inorganic surface technology that forms a barrier between the sample and the metal surfaces of both the HPLC system and chromatographic column. Formed by a vapor deposition of an ethylene-bridged siloxane polymer on metal substrates), this technology effectively addresses common chromatographic challenges such as analyte loss, carryover, and peak tailing due to metal-analyte interactions. It improves peak symmetry and areas, as well as reproducibility, thereby not only benefiting challenging analytes but also increasing confidence in analytical results. We demonstrate the benefits of this technology through the analysis of B-group vitamins, steviol glycosides, and dextran oligosaccharides typically found in food, beverages, and dietary supplements, showcasing its critical role in improving chromatographic performance.