Instrumental Innovations: July/August 2023

Researchers from Bristol Myers Squibb and Sanofi recently tested a new means of using hydrophobic interaction chromatography (HIC) for removing impurities in monoclonal antibodies (mAbs).

This paper describes the content of a well-written analytical procedure for regulated high-performance liquid chromatography (HPLC) testing. A stability-indicating HPLC assay for a drug product illustrates the required components for regulatory compliance, including additional parameters to expedite a laboratory analyst’s execution.

The article discusses the application of a hybrid organic/inorganic surface technology that forms a barrier between the sample and the metal surfaces of both the HPLC system and chromatographic column. Formed by a vapor deposition of an ethylene-bridged siloxane polymer on metal substrates), this technology effectively addresses common chromatographic challenges such as analyte loss, carryover, and peak tailing due to metal-analyte interactions. It improves peak symmetry and areas, as well as reproducibility, thereby not only benefiting challenging analytes but also increasing confidence in analytical results. We demonstrate the benefits of this technology through the analysis of B-group vitamins, steviol glycosides, and dextran oligosaccharides typically found in food, beverages, and dietary supplements, showcasing its critical role in improving chromatographic performance.

In this column, we look at the current version and the update of USP <621> on high-performance liquid chromatography (HPLC) that becomes effective 1st May 2025.

At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to Brett Paull from the University of Tasmania and HyTECH about his talk entitled: “Analytical Performance and Disruptive Potential of Fully Portable Portable and Field Deployable LC–MS Technology.”

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