Simultaneous Determination of Methotrexate and Sulphasalazine in Plasma by HPLC–DAD
May 1st 2015Here we describe a simple and robust high performance liquid chromatography–diode array detection (HPLC–DAD) method for the simultaneous determination of methotrexate (MTX) and sulphasalazine (SSZ) from plasma. MTX and SSZ are used in combination for the treatment of rheumatoid arthritis. Using two detector wavelengths, 304 nm for MTX and 358 nm for SSZ, we were able to selectively quantitate both analytes during the same chromatographic run. The method was validated using quality control samples for critical analytical performance criteria of recovery, reproducibility, selectivity, accuracy, and precision.
A New Era for Lab-on-a-Chip Technologies?
April 24th 2015Miniaturization has been one of the biggest trends in recent years. This article discusses the evolution and current applications of lab?on-a-chip technology in chromatography and explores the possibility of a new market for microfluidics in separation science.
Benzodiazepine and Z-Drug Quantitation Using an Agilent 6430 LC-MS-MS
April 16th 2015A method was developed for the quantitation of benzodiazephines in biological samples using an Agilent 6430 Triple Quadrupole LC/MS system. The method displays excellent accuracy and precision using a weighted-quadratic calibration model.
Validation of a Benzodiazepine and Z-Drug Methods Using an Agilent 6430 LC-MS-MS
April 16th 2015Validation studies showed that the LC/MS/MS method provides reliable results for the analysis of benzodiazephines and the z-drugs zolpidem, zopiclone and zaleplon that meet acceptable criteria set for this application.