Application Notes

Looking to switch LC columns for pharmaceutical drug applications? Method transfer from Luna columns is made seamless with the targeted selectivity of Sonoma LC columns.

A quick reference chart explaining allowable changes to isocratic and gradient HPLC methods

Symmetrical, well-resolved peaks easily meet USP performance requirements for the analysis of fluconazole.

Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.

Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.

This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.

The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.

The analysis of FMOC-derivatized (fluorenmethyloxycarbonyl) glyphosate and AMPA according to DIN ISO 16308 is performed using a YMC-Triart C18 UHPLC column at pH 9.5.

This application note is the first reported separation of Fluoxetine on polysaccharide-based chiral HPLC columns, and it comes on Daicel’s newest column, CHIRALPAK® IK.

Discover the BeatBox platform: fast and straightforward workflow suitable for high-throughput homogenization of challenging tissues for deeper proteomic analyses.

This application note presents RSDs of phthalates with a reference polymer material for IEC 62321-8 method using a CDS 6000 Series Pyroprobe Autosampler.

Custodion® Coiled Microextraction syringes and the Torion® T- 9 Portable GC–MS are fast, easy-to-use screening tools for drugs of abuse and new psychoactive substances.

The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.

Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

This validation study demonstrates the use of the QSight® tandem quadrupole mass spectrometer (LC–MS/MS) for the application of PFAS EPA Method 537.1 in drinking water.

This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.

In this application note, an HPLC with MWD detection method is detailed to detect parabens in cosmetic and personal care products.

This application note details two LC–MS/MS methods for the analysis of 30 analytes from veterinary drugs in milk.

Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity

Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).

This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.

A simple, fast, robust green chemistry method for the accurate quantitation of six additives in diet soft drinks using HPLC with photodiode array (PDA) detection.

NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

This application note highlights the use of solid-phase extraction (SPE) coupled with LC–MS/MS for sample enrichment and quantitation of endocrine-disrupting chemicals.

This application note describes a rapid LC–MS/MS method for the separation and detection of 44 drug of abuse compounds in urine samples.

SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.