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Application Note: The Chiral Resolution of Fluoxetine
This application note is the first reported separation of Fluoxetine on polysaccharide-based chiral HPLC columns, and it comes on Daicel’s newest column, CHIRALPAK® IK.
BeatBox: High-throughput Tissue Homogenization
Discover the BeatBox platform: fast and straightforward workflow suitable for high-throughput homogenization of challenging tissues for deeper proteomic analyses.
White Paper - Evaluation of hydrogen as a carrier gas
Phthalate Analysis with a Reference Polymer
This application note presents RSDs of phthalates with a reference polymer material for IEC 62321-8 method using a CDS 6000 Series Pyroprobe Autosampler.
In-Field Screening for Fentanyl Analogs Using CME and Portable GC–MS v2
Custodion® Coiled Microextraction syringes and the Torion® T- 9 Portable GC–MS are fast, easy-to-use screening tools for drugs of abuse and new psychoactive substances.
Aggregate Analysis of Recombinant Human Growth Hormone v2
The SEC separation of recombinant human growth hormone somatotropin with good peak shape and separation of high molecular weight aggregate was observed.
White Paper: Size-Exclusion Chromatography (SEC) for the Determination of Protein Hydrodynamic Radius
Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.
Analysis of PFAS in Drinking Water with EPA Method 537.1 Using QSight UHPLC–MS/MS
This validation study demonstrates the use of the QSight® tandem quadrupole mass spectrometer (LC–MS/MS) for the application of PFAS EPA Method 537.1 in drinking water.
The Effect of Column Internal Diameter for Robust SEC Methods
This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.
Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection
In this application note, an HPLC with MWD detection method is detailed to detect parabens in cosmetic and personal care products.
Determination of Antibiotics and Veterinary Drugs in Milk by QSight LC–MS/MS
This application note details two LC–MS/MS methods for the analysis of 30 analytes from veterinary drugs in milk.
Aggregate Analysis of an IgG2 Monoclonal Antibody Using a Biozen dSEC-2 LC Column
Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity
Improving Resolution for Size-Exclusion Chromatography Methods
Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column
Assessing Column Lifetime of a UHPLC Biozen dSEC-2 LC Column
The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).
Organic Solvent in Size-Exclusion Chromatography of an Antibody Drug Conjugate Surrogate
This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.
A Fast, Simple, and Green Chemistry Method for the Analysis of Additives in Diet Soft Drinks by HPLC
A simple, fast, robust green chemistry method for the accurate quantitation of six additives in diet soft drinks using HPLC with photodiode array (PDA) detection.
Batch-to-Batch Comparison of Sub-2 µm Diol-Linked Size-Exclusion Columns
NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.
Estrogen Monitoring in River Water by LC/MS/MS with Online SPE v2
This application note highlights the use of solid-phase extraction (SPE) coupled with LC–MS/MS for sample enrichment and quantitation of endocrine-disrupting chemicals.
Fast Screening 44 Illicit Drugs by UHPLC–MS/MS v2
This application note describes a rapid LC–MS/MS method for the separation and detection of 44 drug of abuse compounds in urine samples.
SEC–High Resolution Mass Spectrometry (HRMS) for IdeZ Digested Monoclonal Antibody
SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.
Analysis of United States Pharmacopeia (USP) Grade Isopropyl Alcohol Impurities
This application note describes two methods for isopropyl alcohol impurities detection: USP recommended 42-min analysis, and a speed-optimized method in less than 6 min.
Unispray: An Alternative Ionization Source for trace detection
UniSpray is a novel atmospheric ionization technique that provides increased peak response, enabling ultra-trace detection of PFAS and other contaminants.
On the Chapter 2.1.7 “Balances for analytical purposes” of the European Pharmacopoeia (Ph.Eur.)
Use of laboratory balances in the pharmaceutical industry
Application note: Reversed-Phase Flash Chromatography Purification of Peptide-Peptoid Hybrids
Purification of peptide-peptoid hybrids can be obtained by reversed-phase chromatography with excellent recovery and resolution.
Application note: Improved Compound Recovery with ELSD Systems
The ability to collect compounds is related to the ability to detect them. Many possess weak or non-existent chromophores, causing UV detection to exhibit poor yields.
Purification of Single-Stranded RNA Oligonucleotides
Purification of a single-stranded RNA oligonucleotide by ion pair reversed-phase HPLC is demonstrated, achieving > 99% pure fraction pool with a yield of > 56%.
The Chromatography & Sample Preparation Terminology Guide
Regulatory Considerations in Pharmaceutical Analysis and Implications for LC Method Development
High-Throughput Intact Mass Analysis of mAb-Based Therapeutics