Application Notes

The hydrolysis and SPE steps were combined for designer and pharmaceutical benzodiazepines from urine along with a fast quantitative LC–MS/MS method.

A low-level extraction of steroids using Novum PRO SLE is performed and compared with another SLE product for the baseline cleanliness.

ZipChip CE-MS offers a fast and easy method for native charge variant analysis and product monitoring workflows that are often challenging with traditional LC-MS.

A variety of C18-type columns are evaluated for the identification of levetiracetam via the USP monograph.

Symmetrical, well-resolved peaks easily meet USP performance requirements for the analysis of levetiracetam impurities.

Symmetrical, well-resolved peaks easily meet USP performance requirements for the analysis of pramipexole dihydrochloride.

Easily meet USP method requirements with reduced backpressure using a larger ID column.

A variety of C18-type columns are evaluated for the identification of hydrocortisone via the USP monograph.

The pharma industry is embracing the challenge of sustainability by using greener solvents. A GC–MS method for analyzing 32 green solvents is presented.  

Looking to switch LC columns for pharmaceutical drug applications? Method transfer from Luna columns is made seamless with the targeted selectivity of Sonoma LC columns.

A quick reference chart explaining allowable changes to isocratic and gradient HPLC methods

Symmetrical, well-resolved peaks easily meet USP performance requirements for the analysis of fluconazole.

Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.

Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.

This application note outlines the fractionation of hydrocarbons into aliphatic and aromatic fractions using UCT’s new silver-phase EPH fractionation cartridges.

The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.

The analysis of FMOC-derivatized (fluorenmethyloxycarbonyl) glyphosate and AMPA according to DIN ISO 16308 is performed using a YMC-Triart C18 UHPLC column at pH 9.5.