Application Notes

The characteristic flexibility, stability, high throughput, and fast time to report analytical results of the Syft Tracer next-generation SIFT-MS instrument apply across multiple headspace approaches for diverse matrices. This application note briefly summarizes the use of (1) dissolution, (2) multiple headspace extraction (MHE), and (3) the method of standard additions, then provides a guide for identifying the appropriate headspace approach for various matrices.

The power of vacuum ultraviolet (VUV) light absorption can be useful in problematic moisture determination areas. A new detector for gas chromatography (GC) based on VUV absorption can potentially detect ultra-trace levels of moisture in acetonitrile.

Here we introduce LC/MS analysis methods using the Shodex™ HILICpak™ VC-50 2D column for various cationic neurotransmitters.

Complete and clean trypsin digestion can be achieved with an enzyme that provides a balance of low levels of missed cleavage, non-specific cleavage, and trypsin autolysis. RapiZyme, a next generation trypsin, offers efficiency and reproducibility.

The HILICpak™ VG-50 series columns are able to achieve high recovery rates of saccharides, organic acids, and amino acids.

Hydrophilic Interaction Liquid Chromatography (HILIC) can provide better separation for oligosaccharide analysis than Size Exclusion Chromatography (SEC) mode does.

This application demonstrated the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various sugars found in Japanese sake.

Thermal desorption GC-MS is used to quantify indoor limonene concentrations from diffusers.

Mucopolysaccharidosis and oligosaccharidosis are LSDs caused by enzyme deficiencies. Early diagnosis is key using chromatographic separation and MS/MS identification.

Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).

Demonstration of the sensitivity gain for the Pierce peptide mix, which is achieved when using UHPLC columns of different i.d.s, as well as a micro-LC approach.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

A siRNA duplex and its single strands are separated using different YMC size exclusion columns to identify the most suitable SEC column.

Reversed-phase LC–MS analysis of two phospholipids—LPC (16:0) and PC (34:2)—using a bioinert coated column in comparison with a regular stainless steel one.

Different oligonucleotide mixtures were analyzed using the bioinert coated column YMC-Accura Triart Diol-HILIC in comparison to the conventional stainless steel column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column. The column provides high intensities, recoveries, and great peak shapes.

Analysis of denatured and non-denatured siRNA by use of the bioinert YMC-Accura Triart Bio C18 column in IP-RP mode and the BioPro IEX QF column in AEX mode.

This application note demonstrates that the Agilent 6475 triple quadrupole LC/MS system can confidently quantify nitrosamine impurities at the low concentration levels specified by regulatory requirements. This method can be used to quantify these impurities in different ARB drug products, with some changes in chromatographic conditions based on the elution pattern of the drug product.

Introducing InfinityLab Poroshell 120 Aq-C18 columns—the latest addition to Agilent's Poroshell 120 column portfolio. With InfinityLab Poroshell 120 Aq-C18 columns, you can now maximize reversed-phase retention for highly polar analytes with 100% aqueous mobile phases and develop separations that retain polar and nonpolar compounds togethe

A group of polar compounds was separated on a newly developed Agilent InfinityLab Poroshell 120 Aq-C18 column. The retention loss due to pore dewetting was investigated by comparing the new InfinityLab Poroshell 120 Aq-C18 column to the current C18 phase and other superficially porous columns from various manufacturers

This application note demonstrates improved performance when separating polar compounds using Agilent's InfinityLab Poroshell 120 Aq-C18 column

This application note demonstrates the analysis of ten vitamin B compounds plus ascorbic acide and caffeine using Agilent's InfinityLab Poroshell 120 Aq-C18 column.

This application note presents a side-by-side comparison of the AdvanceBio and GlycoWorks kits for the preparation and analysis of released N-glycans.

Tyrosine Kinase Inhibitors (TKIs) are a class of small molecule drugs used to alter cell growth pathways for the treatment of cancer. Specifically, Sunitinib, a TKI on the market, is available to treat a variety of cancers including pancreatic cancer. Here we create a method to support the therapeutic drug monitoring of Sunitinib and its active metabolite.