Application Notes

Mucopolysaccharidosis and oligosaccharidosis are LSDs caused by enzyme deficiencies. Early diagnosis is key using chromatographic separation and MS/MS identification.

Columns of the YMC-Pack Diol SEC series (120–300 Å), which have distinct pore sizes, were tested for their ability to separate DNA of various lengths (10–300mer).

Demonstration of the sensitivity gain for the Pierce peptide mix, which is achieved when using UHPLC columns of different i.d.s, as well as a micro-LC approach.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

A siRNA duplex and its single strands are separated using different YMC size exclusion columns to identify the most suitable SEC column.

Reversed-phase LC–MS analysis of two phospholipids—LPC (16:0) and PC (34:2)—using a bioinert coated column in comparison with a regular stainless steel one.

Different oligonucleotide mixtures were analyzed using the bioinert coated column YMC-Accura Triart Diol-HILIC in comparison to the conventional stainless steel column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column. The column provides high intensities, recoveries, and great peak shapes.

Analysis of denatured and non-denatured siRNA by use of the bioinert YMC-Accura Triart Bio C18 column in IP-RP mode and the BioPro IEX QF column in AEX mode.

This application note demonstrates that the Agilent 6475 triple quadrupole LC/MS system can confidently quantify nitrosamine impurities at the low concentration levels specified by regulatory requirements. This method can be used to quantify these impurities in different ARB drug products, with some changes in chromatographic conditions based on the elution pattern of the drug product.

Introducing InfinityLab Poroshell 120 Aq-C18 columns—the latest addition to Agilent's Poroshell 120 column portfolio. With InfinityLab Poroshell 120 Aq-C18 columns, you can now maximize reversed-phase retention for highly polar analytes with 100% aqueous mobile phases and develop separations that retain polar and nonpolar compounds togethe

A group of polar compounds was separated on a newly developed Agilent InfinityLab Poroshell 120 Aq-C18 column. The retention loss due to pore dewetting was investigated by comparing the new InfinityLab Poroshell 120 Aq-C18 column to the current C18 phase and other superficially porous columns from various manufacturers

This application note demonstrates improved performance when separating polar compounds using Agilent's InfinityLab Poroshell 120 Aq-C18 column

This application note demonstrates the analysis of ten vitamin B compounds plus ascorbic acide and caffeine using Agilent's InfinityLab Poroshell 120 Aq-C18 column.

This application note presents a side-by-side comparison of the AdvanceBio and GlycoWorks kits for the preparation and analysis of released N-glycans.

Tyrosine Kinase Inhibitors (TKIs) are a class of small molecule drugs used to alter cell growth pathways for the treatment of cancer. Specifically, Sunitinib, a TKI on the market, is available to treat a variety of cancers including pancreatic cancer. Here we create a method to support the therapeutic drug monitoring of Sunitinib and its active metabolite.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column, providing high intensities, recoveries, and great peak shapes.

Detecting over 120 pollutants including pesticides, PAHs, PCBs, phthalates and adipates, this method can prove useful in environmental crises involving water pollution.

Analysis of trace per- and polyfluorinated organic vapours in air using cryogen-free thermal desorption and gas chromatography–mass spectrometry

This application note examines a group of UHPLC columns and their analytical conditions using synthesized miRNA samples.

This application note describes online monitoring of a chemical reaction that produces a byproduct, and presents a fully automated sampling workflow.

Learn how a single method was developed for simultaneous determination of two active ingredients, replacing several separate methods for the analysis of assays and organic impurities.

This application note shows that a single column to be used for the analysis of a wide range of molecular sizes, from intact proteins and monoclonal antibodies to digested peptides.

Here, we introduce HPLC columns with wider pores designed to analyze the characterization of the first-dimensional structure of peptides in reverse phase condition.

Application of C18, 1.6 µm UHPLC columns in the analysis of insulin with strong and weak ion pairing agents to see how they increase the height of the insulin peak.