Application Notes

A rapid, automated polymer degradation study of polymers containing UV stabilizers under ultraviolet (UV) light in a temperature-controlled environment.

Empore StageTips was widely used in peptides and proteins desalting in Proteomics. The sample loading amount on total number of protein identifications was evaluated.

This application note presents data on a rapid, automated polymer degradation study of rubber under ultraviolet (UV) light in air.

SweetSep is a new line of HPAEC columns from Antec Scientific for superior separation of all classes of carbohydrates including N-glycans using PAD or MS detection.

Reliable and reproduceable liquid handling results depend crucially on the regular test and calibration of the instruments. The globally most accepted International Standardization Organization (ISO) standard that details requirements for producing and in-use control of piston-operated volumetric apparatus (POVA) is the ISO 8655. The aim of this white paper is to present the requirements for gravimetric calibration and testing of piston-operated volumetric apparatus (POVA) according to part 6 and part 7 of the ISO 8655 revised in 2022. This white paper presents the differences between calibration and testing according to chapters 6 and 7 , describes the testing method as well as testing environment, testing equipment and reporting requirements.

Bulb and graduated pipettes made of glass or plastic are common in the laboratory, especially when pipetting volumes greater than 1,000 µL. One example of graduated pipettes are serological pipettes. Plastic (disposable) serological pipettes are widely used in cell culture applications. There are multiple reasons to replace serological pipettes with Sartorius pipettes. Discover the three main reasons to switch to a Sartorius pipette.

ISO 8655:2022 contains updated requirements for producing and in-use control of piston-operated volumetric apparatus (POVA), replacing the 1st edition from 2002. ISO 8655:2022 consists of nine parts in total. Here, we describe the main points about Part 2: Pipette

A disadvantage of modern LIBs is instability of the electrolytes. Learn about the use of IC for analysis of decomposition products to evaluate electrolyte degradation.

Pipettes and pipette accessories are staples in most laboratory environments, from biochemistry to pharmaceuticals to beverage quality control. Because different assays have different needs in terms of equipment and technique, it is important to assess the particular set of needs required in your lab. Key requirements for most modern-day applications include: Accuracy, minimizing human errors, speed, easy-to-clean-instruments, precision, ability to decontaminate and avoiding contamination.

In this guide, Sartorius explores some factors to consider when choosing a manual or electronic pipette for microbiological analyses. Requirements for liquid handling in a microbiology lab might be different from other analytical laboratories. Here are some points to consider before committing to purchasing a pipette

This app note demonstrates a comprehensive software workflow for non-targeted analysis of per- and polyfluoroalkyl substances by high-resolution mass spectrometry.

In this app note, we evaluate the performance of the novel Orbitrap Astral mass spectrometer for plasma proteomics using 4 of the most common plasma sample prep methods

Clinical Research: Streamlined workflow and minimum up-front development efforts using the first commercial ready-to-use kits for quantitative analysis of mAbs

Comparison of analytical methods for quantification of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 on a Thermo Scientific™ TSQ Altis™ MD mass spectrometer

This tech note presents a low-input proteomics sample workflow using the Pierce HeLa protein digest standard in nanogram and picogram loads on the Orbitrap Astral mass spectrometer

This application note discusses an advanced sensitive, reproducible and efficient single-cell measurement and sample throughput workflow platform.

Thermo Scientific ProPac 3R SAX columns provide excellent separation of Protein G charge variants using a salt gradient. Its unique design delivers high resolution, robust performance, and excellent reproducibility needed for charge variant analysis of proteins.

Designed with bio-inert materials, the new Thermo Scientific ProPac 3R columns support the delivery of reproducible, high resolution and robust strong anion and strong cation exchange chromatography separations for protein and monoclonal antibody analysis.

With advances in the technologies used in LC-MS-based proteomics workflows, sensitive, reproducible, and efficient single-cell proteomics is now possible. Learn about these advances and how they work in concert to maximize the combination of protein and peptide identifications and sample throughput.

ProPac 3R SAX columns will provide high resolution, robust performance and unrivalled lot-to-lot reproducibility needed for these applications.

Thermo Scientific ProPac 3R SCX columns provide excellent separation of mAb and associated charge variants using a salt gradient to give high-resolution, robust performance and excellent reproducibility

Thermo Scientific ProPac 3R SCX columns, combined with Thermo Scientific CX-1 pH gradient buffers, provide fast, reproducible, and ultra-high resolution for monoclonal antibodies and associated charge variants.

Nine structurally diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient.

Chemically resistant resin bed allows for a wide base of mobile phase choices with excellent anion separation

This focuses on the identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

The isolation of the diterpenoid, paclitaxel, from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

A reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.