Application Notes

Although the majority of benzodiazepines metabolize to a single compound, oxazepam, other metabolites can be detected by liquid chromatography.

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.

Paclitaxel originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).

A Hamilton PRP-C18 (5 μm) HPLC column was used for purification of three oligonucleotides. Analysis of a 21-mer, 22-mer, and 23-mer of similar structure was evaluated.

The new Ascentis® Express PFAS HPLC and delay columns allow the highly efficient separation of 33 PFAS compounds in 5 minutes with reduced background contamination.

The presented methods are suitable for the quantification of THC and THCA (and other cannabinoids) in Cannabis sativa and to check THC-free cannabis for compliance.

This application note introduces a simple targeted extraction method for the analysis of nine nitazene compounds from urine and blood using the Clean Screen® DAU column.

The primary nucleosides encompass a broad array of biological functions associated with RNA, particularly after configuration into multidimensional conformations.

The nutrition profile of food is critical for assessing its quality. In this poster the amino acids in soy flour were hydrolyzed using a Discover Prep microwave system.

Plant-based proteins are becoming a mainstay to grocery stores, restaurants, and retailers. Amino acids in vegan cream cheese are herein hydrolyzed and analyzed.

In this app note, we perform amino acid hydrolysis of proteins to identify and quantify each amino acid utilizing a safe and rapid amino acid hydrolysis protocol.

Protein analysis plays a large role in the production of foods, pet foods, and even pharmaceutical medicines. But first, the amino acids in the protein must be broken down.

Peptide therapeutics are held to high regulatory standards. Discover Prep was used to hydrolyze the amino acids of a peptide in only 15 minutes, instead of 24 hours.

In this study, SPME Arrow sample preparation was tested for the analysis of furan and alkylfurans in baby formula and coffee samples.

Classical protocol of amino acid hydrolysis needs up to 24 h of reaction time. Using the Discover Prep, the same level of acid and base hydrolysis was achieved in minutes.

This application note describes a 2D-LC workflow for Protein A purification of mAbs, followed by online reduction and MS analysis of mAb subunits.

The determination of boiling range distribution of middle distillates and lubricating base oils by GC, with initial IBP of 100°C and FBP of below 750°C.

This is the final in a series of three eBooks discussing and offering practical advice on the journey from current ways of working to a digital laboratory operating in a regulated environment. Learn more about:

A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column.

A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.