All
Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF
June 20th 2023Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.
MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography
June 20th 2023In this application note, the determination of oligo sequence confirmation using HILIC LC and high-resolution MS/MS data is described. As with the previous studies, an InfinityLab Poroshell 120 HILIC-Z column was used along with an Agilent 6545XT AdvanceBio LC/Q-TOF mass spectrometer.
MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography
June 20th 2023In this application note, LC separation and MS1 mass identification of a variety of oligos without the use of ion‑pairing reagents is demonstrated. The LC separation allows subsequent positive mode use with little to no flushing or hardware changes. This HILIC-based method uses an Agilent InfintyLab Poroshell 120 HILIC-Z column and MS-friendly ammonium acetate-based mobile phases. The samples were analyzed on an Agilent 1290 Infinity II LC system and a 6545XT AdvanceBio quadrupole time-of-flight mass spectrometer (LC/Q-TOF).
Gravimetric Calibration and Testing of POVA (ISO8655:2022)
June 7th 2023Reliable and reproduceable liquid handling results depend crucially on the regular test and calibration of the instruments. The globally most accepted International Standardization Organization (ISO) standard that details requirements for producing and in-use control of piston-operated volumetric apparatus (POVA) is the ISO 8655. The aim of this white paper is to present the requirements for gravimetric calibration and testing of piston-operated volumetric apparatus (POVA) according to part 6 and part 7 of the ISO 8655 revised in 2022. This white paper presents the differences between calibration and testing according to chapters 6 and 7 , describes the testing method as well as testing environment, testing equipment and reporting requirements.
Three Main Reasons to Choose Sartorius Pipettes and Pipette Tips Instead of Serological Pipettes
June 7th 2023Bulb and graduated pipettes made of glass or plastic are common in the laboratory, especially when pipetting volumes greater than 1,000 µL. One example of graduated pipettes are serological pipettes. Plastic (disposable) serological pipettes are widely used in cell culture applications. There are multiple reasons to replace serological pipettes with Sartorius pipettes. Discover the three main reasons to switch to a Sartorius pipette.
ISO 8655:2022 and the Pipette-Tip System
June 7th 2023ISO 8655:2022 contains updated requirements for producing and in-use control of piston-operated volumetric apparatus (POVA), replacing the 1st edition from 2002. ISO 8655:2022 consists of nine parts in total. Here, we describe the main points about Part 2: Pipette
Basics of Pipetting Handbook - Your Guide to Getting the Most Out of Your Pipette
June 7th 2023Pipettes and pipette accessories are staples in most laboratory environments, from biochemistry to pharmaceuticals to beverage quality control. Because different assays have different needs in terms of equipment and technique, it is important to assess the particular set of needs required in your lab. Key requirements for most modern-day applications include: Accuracy, minimizing human errors, speed, easy-to-clean-instruments, precision, ability to decontaminate and avoiding contamination.
How to Choose a Microbiology Laboratory Pipette
June 7th 2023In this guide, Sartorius explores some factors to consider when choosing a manual or electronic pipette for microbiological analyses. Requirements for liquid handling in a microbiology lab might be different from other analytical laboratories. Here are some points to consider before committing to purchasing a pipette