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A Comprehensive Workflow for PFAS Analysis in Wastewater with Extended EPA Draft Method 1633 List
July 6th 2023This study assesses a workflow developed for PFAS analysis in wastewater, based on the existing EPA draft Method 1633 with additional PFAS of varying size and functional group. This workflow contains off-line solid phase extraction (SPE) and a clean-up step, followed by LC-MS/MS analysis, and automatic reporting using SLIMS. The workflow demonstrates a reliable solution for the targeted analysis of PFAS in complex matrices with high robustness.
Building an Efficient Cannabis Testing Lab that Meets Compliance Standards
June 29th 2023In an ever-evolving cannabis industry where regulatory compliance and accurate testing are paramount, staying ahead requires a deep understanding of how to build an efficient testing lab that meets compliance standards:
Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF
June 20th 2023Characterization of oligonucleotides requires robust analytical instrumentation and methods as well as ease-of-use data analysis tools. Biocompatibility mitigates non-specific sample binding to flow path and it ensures the integrity of biomolecules and robustness of the system. In this study, two workflows, the Target Plus Impurities (TPI) and Sequence Confirmation workflows in Agilent MassHunter BioConfirm software, were carried out to characterize two oligonucleotide samples.
MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography
June 20th 2023In this application note, the determination of oligo sequence confirmation using HILIC LC and high-resolution MS/MS data is described. As with the previous studies, an InfinityLab Poroshell 120 HILIC-Z column was used along with an Agilent 6545XT AdvanceBio LC/Q-TOF mass spectrometer.
MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography
June 20th 2023In this application note, LC separation and MS1 mass identification of a variety of oligos without the use of ion‑pairing reagents is demonstrated. The LC separation allows subsequent positive mode use with little to no flushing or hardware changes. This HILIC-based method uses an Agilent InfintyLab Poroshell 120 HILIC-Z column and MS-friendly ammonium acetate-based mobile phases. The samples were analyzed on an Agilent 1290 Infinity II LC system and a 6545XT AdvanceBio quadrupole time-of-flight mass spectrometer (LC/Q-TOF).