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Salt gradient analysis of monoclonal antibodies using a 3 μm monodisperse SCX column
Thermo Scientific ProPac 3R SCX columns provide excellent separation of mAb and associated charge variants using a salt gradient to give high-resolution, robust performance and excellent reproducibility
Method development for pH gradient analysis
Thermo Scientific ProPac 3R SCX columns, combined with Thermo Scientific CX-1 pH gradient buffers, provide fast, reproducible, and ultra-high resolution for monoclonal antibodies and associated charge variants.
HPLC 2023: Hot Topics in (U)HPLC
Separation of Strongly Basic Drug Compounds (May 2023)
Nine structurally diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient.
Flexible Anion Analysis with Hamilton Columns
Chemically resistant resin bed allows for a wide base of mobile phase choices with excellent anion separation
Spill the Tea, Catechin Identification (May 2023)
This focuses on the identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.
Pick Your Poison. Isolation of Paclitaxel (May 2023)
The isolation of the diterpenoid, paclitaxel, from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
Separation of Five Steroids (May 2023)
A reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).
Separation of Basic Drug Compounds (May 2023)
In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.
Separation of Selected Ylang-Ylang Oil Components
Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.
Separation of Common Benzodiazepine Metabolites (May 2023)
The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.
Advances in Mass Spec Instrument Intelligence
DOW Designs Efficiencies into Method Development
This application note describes how Dow AgroSciences created efficiency in method development by incorporating method development software into their best practices.
Quantification and Identification of Microplastics in Air Using Pyrolysis-GC/MS
Analysis of Glyphosate, Glufosinate, and Metabolites
Outlines an extraction procedure and an optional fluorenylmethyloxycarbonyl chloride derivatization step to allow for the analysis of glyphosate on a C18 column.
Improved Sample Prep of mAbs
Improved and universal subunits LC–MS analysis of multiple classes of monoclonal antibodies using in-line electrochemical reduction
Revolutionizing Volatile Impurities Analysis Through Next Gen SIFT-MS
Chiral Resolution of Sotorasib Atropisomers
Daicel Chiral Technologies' unique HPLC and SFC chiral stationary phases are capable of separating the most challenging compounds, like the atropisomers of Sotorasib.
Smart-Ramped Pyrolysis-GC–MS for Forensic Analysis
Smart-ramped pyrolysis-GC–MS analysis of forensic samples such as paint, adhesive tape, and mascara gives maximum info in a single run without method development.
Smart-Ramped Pyrolysis-GC-MS of Nylon Products
Smart-ramped pyrolysis-GC–MS analysis of nylon material in consumer products, food packaging, and clothing generates info in one run without method development.
Smart-Ramped Pyrolysis-GC–MS of Acrylates and Methacrylates
Smart-ramped pyrolysis-GC–MS analysis of (meth)acrylates in consumer products, caulk, and nail hardener gives maximum info in a single run without method development.
Sampling Plant Volatiles for GC-MS Analysis
Solvent-free extraction by passive sampling using TF-SPME and HSSE, and by active pumped sampling onto sorbent tubes, are compared for TD-GC–MS analysis of volatiles.
Sensory Directed Flavour Analysis of Pea-Based Tuna
Olfactory detection of sensory-active compounds guides GC–MS analysis for efficient flavour assessment of foodstuffs and their plant-based replacement products.
Flavour Analysis of Hard Seltzer Beverages
Solvent-free extraction and analyte concentration using TF-SPME and SBSE are optimized for TD-GC–MS determination of flavour compounds in hard seltzer beverages.
Using Pyrolysis-MS for Evolved Gas Analysis
This study demonstrates the use of pyrolysis-(GC¬–)MS to generate simulated TGA-MS data for polymers. The mode of operation is also referred to as evolved gas analysis.
Microplastics in Water by Smart-Ramped Pyrolysis-GC-MS
Smart-ramped pyrolysis-GC¬–MS analysis of microplastics in water filtration residue gives maximum info in one run. Examples are runoff, grey-, pond-, and bottled water.
Automated Determination of VOC Emissions
A micro-scale chamber method performs DNPH derivatization and Tenax TA sorption for analysis of formaldehyde and carbonyl compounds by LC–UV, and VOCs by GC–MS.
Monoclonal Antibody Analysis (May 2023)
This application note shows that a single column to be used for the analysis of a wide range of molecular sizes, from intact proteins and monoclonal antibodies to digested peptides.
Efficient Pesticide Residue Testing
This application note describes the benefits of automated gravimetric liquid dispensing compared to traditional manual stock solution preparation in a volumetric flask.
UHPLC Columns for Nucleic Acid Oligomer Analysis (May 2023)
This application note examines a group of UHPLC columns and their analytical conditions using synthesized miRNA samples.