Application Notes: LC

The fast simple method developed by Hamilton Company using the PRP-C18 column allows for simple, efficient identification of the whole vitamin series (K2, K3, and K4).

A Hamilton PRP-C18 (5 μm) HPLC column was used for purification of three oligonucleotides. Analysis of a 21-mer, 22-mer, and 23-mer of similar structure was evaluated.

The primary nucleosides encompass a broad array of biological functions associated with RNA, particularly after configuration into multidimensional conformations.

This application note describes a 2D-LC workflow for Protein A purification of mAbs, followed by online reduction and MS analysis of mAb subunits.

A separation of parabens is performed on a HALO 90 Å LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column.

A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.

The natural products of the cannabis plant can number up to 100, but the scientific community is concerned with a smaller portion of around 10 to 20 compounds.

A low pH compatible, 90 Å, superficially porous particle C18 phase is introduced as an addition to the chromatographer’s toolbox.

A HALO® LPH-C18 column is used to separate a mixture of 18 cannabinoids, showing fast results and high resolution within critical pairs.

The fast identification of tocopherols is important for various applications including free radical scavenging for human health and food stuff preservation.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

Explore how a systematic screening strategy allows chromatographers to rapidly identify ideal method conditions.

This white paper explores the theory behind solid-core particles and the advantages they offer for small molecule analysis.

This article discusses the impact and theory of column temperature and how it can be a powerful tool for fine-tuning a separation during method development.

An increased number of samples demands increased throughput. This article discusses ways to achieve high throughput analysis with LC–MS analysis methods.

In LC separations, the majority of analytes are ionizable, so controlling the state the analytes are in is critical. Discover how to harness pH for method development.

In this app note, a two-dimensional separation is conducted on an oxidative lignin depolymerization mixture using centrifugal partition chromatography.

The fast identification of tocopherols are important for various applications including free radical scavenging for human health and food stuff preservation.

The use of resistor tubing and 200 mM ammonium acetate in the mobile phase was implemented to measure loading capacity of a Biozen 1.8 μm dSEC-2 column.

Protein standards were explored with known hydrodynamic radii, showing how this can be used to determine ideal size exclusion conditions for optimal method performance.

Using standard phosphate containing buffers with different percentages of isopropanol for aggregate analysis of an antibody-drug conjugate (ADC), Kadcyla

Temperature, gradient program, and flow rates are explored and optimized for intact mass applications for mAbs when using Biozen WidePore C4 column with bioTi hardware.

The hydrophilic nature of the Biozen dSEC-2 stationary phase shows favorable aggregate profiles for forced degradation work under high salt conditions.

A robust and sensitive HPLC method with multi-wavelength UV–vis detector for the analysis of 2-phenoxyethanol and seven parabens in cosmetic and personal care products.

Molnupiravir, which is a prodrug, and its active agent β-d-N4-hydroxycytidine can be separated by using a YMC-Triart C18 UHPLC column and a MS-compatible mobile phase.

This work introduces the analysis of iso-α-acids, α-acids, and humulinones in beer in reference to EBC 9.47. IBU was also measured with a UV–vis spectrophotometer.

In this app note, xanthohumol, isoxanthohumol, humulinones, iso-α-acids, α-acids, and β-acids are simultaneously analyzed by high performance liquid chromatography.

This article introduces the high-speed analysis of α-acids and β-acids in hops in reference to EBC 7.7 and ASBC Hops-14.

Volatile sulfur compounds were analyzed in commercially available beer samples using GC with headspace sampler and sulfur chemiluminescence detection.

Shimadzu provides total solutions for beer analysis from farm to stein and service support to keep up with changing market demands.