Application Notes: LC

Hamilton's PRP-C18 column gave good separation with various gradient profiles and under various particle sizes. The excellent loading capacity of the column make this stationary phase desirable.

Modified nucleosides, either natural or synthetic, often provide RNA with distinctive properties useful in diagnostics or therapeutics.

This demonstrates how to perform cannabis potency analysis using HPLC-UV, displaying the advantages of this technique for analyzing acidic cannabinoids.

Accurate determination of flow rate is an increasingly favoured method of assessing or validating the performance of a pump serving a liquid chromatography system.

A fast, simple method using the PRP-C18 column allows for simple, efficient identification of the natural and synthetic compounds of vitamin K (K2, K3, and K4).

Contamination of drinking water and food sources is of great concern. Isolation and quantification of the various arsenic states is identified.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Analyzing crops for beneficial and harmful compounds is an indispensable tool for assessing the viability of various agricultural crops for consumption.

Flavonoids in cannabis, fruits and vegetables protect plants. Hamilton has developed a method to confirm the 7 most common flavonoids in cannabis with the PRP-1 column.

Accurate detection of HAAs is paramount for prevention of overoxidation while still managing enough water sanitation to eliminate water-borne pathogens.

In this application note, we profile capsid protein peptides from AAV5 and AAV9, digested with trypsin using a Biozen™ Peptide XB-C18 reversed phase column.

We present an LC-HRMS approach to distinguishing a pool of multiple caps and their relative integrity of digested mRNA oligonucleotide strands of 1250 nucleotides.

Review profiling of denatured and dissociated capsid proteins from AAV5 and AAV9, via analysis with LC-MS using a Biozen™ Intact XB-C8 reversed phase column.

We present electrical asymmetrical flow field-flow fractionation with multi-angle light scattering (EAF4-MALS) to derive parameters of a liposomal form of doxorubicin.

A separation of antidepressants is compared between a fully porous C18 and the HALO® PCS C18 column.

A synthetic peptide panel is screened on the HALO® Peptide PCS C18 and compared to a standard peptide C18 stationary phase.

Built upon Fused-Core® technology, the HALO® PCS C18 maintains peak symmetry at high loading capacities and provides alternate selectivity for C18 bonded phases.

The new HALO® PCS C18 phase offers an alternative to chromatographic separations.

In a head-to-head SPP comparison of two positively charged C18 surface chemistries, the HALO 90 Å PCS C18 obtains improved resolution, efficiency, and tailing factors.

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

Identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

Nine structurally diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient at 80 °C.

The diterpenoid, paclitaxel, is as a potent chemotherapy agent for breast and ovarian cancer. Here, the isolation of paclitaxel from its major impurities is shown.

Chemically resistant resin bed allows for a wide base of mobile phase choices with excellent anion separation

Identifying benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.