Application Notes: LC

Easily meet USP method requirements with reduced backpressure using a larger ID column.

A variety of C18-type columns are evaluated for the identification of hydrocortisone via the USP monograph.

Looking to switch LC columns for pharmaceutical drug applications? Method transfer from Luna columns is made seamless with the targeted selectivity of Sonoma LC columns.

A quick reference chart explaining allowable changes to isocratic and gradient HPLC methods

Symmetrical, well-resolved peaks easily meet USP performance requirements for the analysis of fluconazole.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

In this application note, an HPLC with MWD detection method is detailed to detect parabens in cosmetic and personal care products.

A simple, fast, robust green chemistry method for the accurate quantitation of six additives in diet soft drinks using HPLC with photodiode array (PDA) detection.

Purification of peptide-peptoid hybrids can be obtained by reversed-phase chromatography with excellent recovery and resolution.

The ability to collect compounds is related to the ability to detect them. Many possess weak or non-existent chromophores, causing UV detection to exhibit poor yields. 

This app note introduces the use of the Nexera XS inert UHPLC, which utilizes a metal-free flow path for accurate and reproducible analysis of nucleotides.  

This app note introduces an analytical method for the separation of oligonucleotides of different length by ion-exchange chromatography using the Nexera XS Inert. 

This study demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent AssayMAP Bravo liquid handling platform.

Peptide-mapping analysis shows excellent performance and high method compatibility compared to the Agilent 1290 Infinity II LC System.

This paper describes the requirements for electronic records and electronic signatures for regulated pharmaceutical organizations.

The 1290 Infinity II Bio LC including High-Speed Pump, with its iron‑free flow path, is optimally suited for the conditions used in biochromatography.

The Agilent Bio-Monolith rProtein A (recombinant protein A) analytical column is the latest addition to the Bio-Monolith and affinity chromatography family.

Analysis of antibody drug conjugates using hydrophobic interaction chromatography with the Agilent 1290 Infinity II Bio LC System

Superior resolution in size exclusion chromatography (SEC) protein separation made possible by the Agilent 1290 Infinity II Bio LC System.

The Agilent 1290 Infinity II Bio LC System is the new platform for UV and MS-based primary structure and PTMs analysis of mAbs.

Accurate detection of halo acetic acids is paramount for the prevention of over oxidation, while still managing enough water sanitation to eliminate water-borne pathogens.

Plasticizers added to water bottles to add stability to the bottles can be unknowingly extracted in drinks leading to possible endocrine interactions. 

This application introduces the automated analysis of fluorescent diastereomers of the proteinogenic D/L-amino acids using derivatizing reagents with chiral structures.

This goal of this note is identity confirmation of protein-based impurities of a therapeutic protein by multi heart-cut 2D-LC–MS in a top-down experiment.

The fight against cancer employs many tools. Paclitaxel, a compound found in the bark of the Pacific yew tree, is one that that has been found to make a big difference.

In this study, we confirm the high selectivity of CPC and its capability to purify a set of key compounds and enrich several minor cannabinoids in a single step.

Exploring isotopes in petrochemical materials can reveal information on original source materials, and maturation and biodegradation processes on different time scales.

Detect aflatoxins B1, B2, G1, and G2 and sterigmatocystin in complex (and simple) sample matrices using a streamlined approach with LC.

Antiretrovirals have come back to the forefront of separation science at this moment in time, as they have found use in the fight against SARS-CoV-2.

The ALEXYS Carbohydrate Analyzer is a dedicated instrument for the routine analysis of sugars and oligosaccharides in F&B products based on HPAEC-PAD.