Application Notes: LC

This tech note describes a method of continuous monitoring of flow rate to provide real-time validation of the LC system performance, improving reliability of results.

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.

Accurate purity assessment of oligonucleotide mixtures can be challenging due to their complexity. Daicel offers several solutions that provide unique selectivity.

Complementarity in chromatography ensures accurate purity. Daicel offers non-chiral stationary phases compatible in different modes for cyclic peptide analysis.

Despite the utility of the essential oil mixture, it has been shown that allergies can arise from some of the individual components.

Nine structurally diverse, strongly basic drug compounds are resolved in less than 2 minutes using a high pH mobile phase and a fast acetonitrile gradient.

Chemically resistant resin bed allows for a wide base of mobile phase choices with excellent anion separation

This focuses on the identification of polyphenolic catechin compounds for health and industrial quantification and quality control of tea products.

The isolation of the diterpenoid, paclitaxel, from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

A reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

In this study, a generic, 5-min linear gradient was used to separate six basic drug compounds on a short (50 mm) PRP-C18 column.

Isolation of six common allergens can be achieved by utilizing reversed-phase HPLC using an alkaline eluent.

The identification benzodiazepine metabolites can be highly valuable in detecting abuse. Metabolites of oxazepam are found containing both hydroxyl and glucuronides.

This brief communication highlights the assessment of Hamilton PRP-3 HPLC column attributes when analyzing biologically active peptides.

This application note demonstrates the performance of multi-detector asymmetrical flow field-flow fractionation for the characterization of macromolecules in beer.

A Hamilton PRP-C18 (5 μm) HPLC column was used for purifying three oligonucleotides. Analysis of a 21mer, 22mer, and 23mer of similar structure were evaluated.

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification and quantification of the Raffinose Family of Oligosaccharides allows a more thorough understanding of an individual’s gut health.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

Isolation of capsaicin for medicinal uses is easily achieved with the PRP-C18 RP-HPLC resin.

Excellent separation and chemical stability in oligonucleotide isolation is observed. The C18 column provides loading capacities and reliability needed for repeat use.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

GRPC peptides are found in the entire body. Isolation of seven classic GRPC peptides initiating in the GI tract could implicate disease.

Hydrophilic Interaction Liquid Chromatography (HILIC) can provide better separation for oligosaccharide analysis than Size Exclusion Chromatography (SEC) mode does.

This application demonstrated the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various sugars found in Japanese sake.

mRNA coupled to EGFP (enhanced green fluorescent protein) is analyzed using a widepore YMC-Accura Triart Bio C4 RP column as well as a non-porous BioPro IEX QF column.

Different oligonucleotide mixtures were analyzed using the bioinert coated column YMC-Accura Triart Diol-HILIC in comparison to the conventional stainless steel column.

Four phosphopeptides are analyzed using a bioinert coated YMC-Accura Triart C18 column. The column provides high intensities, recoveries, and great peak shapes.

This application note demonstrates improved performance when separating polar compounds using Agilent's InfinityLab Poroshell 120 Aq-C18 column

This application note demonstrates the analysis of ten vitamin B compounds plus ascorbic acide and caffeine using Agilent's InfinityLab Poroshell 120 Aq-C18 column.