Application Notes: LC-MS

Automated phosphopeptide enrichment and ion mobility mass spectrometry (IMS-MS) enables detailed analysis of structural effects of site-specific phosphorylation.

Automated Antibody Drug Conjugate (ADC) purification and deglycosylation increases reproducibility and simplifies drug-to-antibody ratio (DAR) determination

Improved host cell protein analysis using highly reproducible and scalable automated sample digestion and fractionation ahead of LC–MS coupled with iterative MS–MS

Easily implement a robust, reliable, and reproducible workflow solution for the analysis and quantitation of nine HAAs, bromate, and dalapon in water using IC–MS/MS.

This application note reveals the Vanquish Neo offers maximal MS utilization for direct injection workflows and versatility to separate peptides at low flow rates.

This application note shows a micro-flow LC–MS method developed on the Vanquish Neo UHPLC is suitable for high-throughput proteomic analyses of large sample cohorts.

ZipChip CE-MS offers a fast and easy method for native charge variant analysis and product monitoring workflows that are often challenging with traditional LC-MS.

Longer chain oligonucleotide therapeutics are more prevalent. The VN-50 2D column successfully separated 10-mer to 50-mer oligonucleotides without ion-pairing.

Phosphorothioated oligonucleotides were successfully separated with the VN-50 2D without ion-pairing and a simple acetonitrile/ammonium formate gradient.

The semi-micro VN-50 1D column contributed to lower diffusion and increased resolution and S/N ratio, necessary for highly sensitive QC analyses of oligonucleotide therapeutics.

The elution patterns of oligo-RNA having ribose modified with 2'-MOE and non-modified oligo-RNA were separated successfully without the use of an ion-pair reagent using the Shodex™ VN-50 2D column.

The analysis of FMOC-derivatized (fluorenmethyloxycarbonyl) glyphosate and AMPA according to DIN ISO 16308 is performed using a YMC-Triart C18 UHPLC column at pH 9.5.

This validation study demonstrates the use of the QSight® tandem quadrupole mass spectrometer (LC–MS/MS) for the application of PFAS EPA Method 537.1 in drinking water.

This application note details two LC–MS/MS methods for the analysis of 30 analytes from veterinary drugs in milk.

This application note highlights the use of solid-phase extraction (SPE) coupled with LC–MS/MS for sample enrichment and quantitation of endocrine-disrupting chemicals.

This application note describes a rapid LC–MS/MS method for the separation and detection of 44 drug of abuse compounds in urine samples.

UniSpray is a novel atmospheric ionization technique that provides increased peak response, enabling ultra-trace detection of PFAS and other contaminants.

This application note features a unique, simple, and robust solid-phase extraction method for cannabinoids in urine followed by analysis by LC–MS/MS.

A novel LC approach to manipulate the chromatography of closely related PFAS analytes to separate co-elutes and avoid matrix interferences.

This note summarises a procedure developed by the US Food and Drug Administration to measure 16 PFAS in food using a QuEChERS sample preparation approach and LC–MS/MS analysis.  

PFAS are known to be persistent in the environment. Detecting low levels of PFAS in drinking water is made easier using an Activated Carbon Delay Column.

The effect of various ion pairing reagents (amines and acidic counterions) on the LC–UV and LC–MS analysis of oligonucleotides (ONs) has been studied.

Use software to deconvolute LC–MS spectra from complex samples and identify unknown components.

With a new electrochemical cell, complete reduction of inter- and intra-disulfide bonds in mAbs is achieved. For cystine knots peptides, the sequence coverage was 100%.

Fused-Core® Technology benefits PFAS analysis for fast, efficient, and rugged separations which are paramount to environmental analysis.

HALO® PFAS and PFAS Delay columns are application verified columns well suited for challenging environmental sample analysis.

High resolution separation of 24 PFAS compounds using the HALO® PFAS Delay column coupled with the HALO® PFAS analytical column.

High throughput analysis of 33 PFAS compounds found across EPA methods 533, 537.1, and 8327 in under 5 minutes.

HALO® PFAS delay and HALO® PFAS analytical columns deliver rugged, reproducible performance for the most challenging environmental sample matrices.

Rapid and high-resolution analysis of PFAS compounds across EPA methods 537.1, 533, and 8327, using the new HALO® PFAS delay and analytical columns.