Application Notes: LC-MS

To overcome peak broadening, this application adjusts the column temperature for the bioZen WidePore C4 to improve peak shape in large molecule reversed-phase applications.

This application analyzes how to reduce peak widths by adjusting the flow rate for intact and reduced NIST mAb when using a bioZen WidePore C4 LC column.

Comparing core-shell superficially porous to fully porous particles to determine efficiency required for detection and separation of low-level impurities for subunit analysis.

Multiple C4 LC columns are compared to determine which provides the necessary separation of the impurity and avoids any spectral overlap in the MS detector.

CE–MS can help address limitations associated with traditional LC–MS CE–MS provides fast separations with low sample consumption and no carry over

- Conventional light scattering comes with various limitations - New light scattering technology benefits protein/polymer characterization

In this study we demonstrate the benefits of the Thermo Scientific™ Orbitrap Exploris™ 240 LC–MS/MS for intact biopharmaceutical analysis under native conditions.

In this application note we evaluate the benefits of Real-Time Search for SPS MS3 (Orbitrap Eclipse MS) to enhance TMT quantification accuracy and proteome coverage.

In this application note we discuss the AcquireX workflow using the Orbitrap ID-X for detection and quantitation in non-targeted, multiresidue LC–MS pesticide profiling.

Microfluidic lab-on-a-chip devices offer new solutions for analytical scientists. The Column spoke to Dietlev Belder from the University of Leipzig, Germany, about the latest developments in this evolving area of research.

PharmaFluidics’ μPAC capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

PharmaFluidics’ μPAC capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

Overall gain in performance and increase in detection sensitivity that can be achieved for the separation of plant metabolite extracts by using µ¬PAC columns.

Reveal more in your food. Using QSight LC–MS/MS to get a fast, selective, and sensitive multi-residue method for the determination of 136 pesticides in pepper.

Method Development for Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy per- and polyfluoroalkyl substances (PFAS).

This application note shows that hydrogen is a valuable alternative to helium, as it is lower in cost and provides great separation in shorter analysis time.

In reversed-phase liquid chromatography, increasing the column temperature usually results in a decrease in analyte retention. Will this cause problems if a method does not call for control of the column temperature but the room temperature of the laboratory environment changes?

HCPs can threaten drug product efficacy and stability. LC–MS/MS using automated sample prep can address HCP analysis challenges. Software and hardware workflow upgrades can optimize HCP analysis methods.

This application demonstrates the efficacy of this chromatographic technique for a fast orthogonal analysis to evaluate mAb ADCC activity.

Learn how Janssen cut method-development time by 80% while improving quality 25-fold by upgrading their hardware and using method-development software.

A simple and sensitive method is presented for the direct analysis of Glyphosate and AMPA in drinking water without sample derivatization or pre-concentration.

This RAFA 2019 winner of the Best Poster Award, reviews the identification and characterization of secoiridoids isomers found in Greek extra virgin olive oil (EVOO).

Automated GC/MS determination of 3-MCPD and glycidol in edible oils. Evaporation step helps reach required LODs with standard MSD and removes excess derivatization reagent for improved stability.

Solid-phase extraction (SPE) is a powerful technique for LC­–MS analysis. SPE offers benefits for various solutes from a wide range of samples. Modern SPE systems can remove unwanted compounds and streamline methods.