Rapid and Accurate Simultaneous Quantitation of Low Concentration Amphetamines and Metabolites from Urine using Strata-X-C and LC–MS–MS on Gemini C18
The use of HPLC for determining amphetamines has not received much attention because of the poor response of UV spectrometric detectors to these compounds when present at low concentration levels. However, HPLC coupled with mass spectrometry can provide the sensitivity required for the analysis of analytes at low concentrations in biological samples. In comparison to GC–MS and LC-UV analyses, LC–MS–MS offers superior sensitivity, selectivity and robustness for a wide range of analytes without any need for derivatization. LC–MS–MS technology also makes possible the development of highly selective, sensitive and precise methods for the simultaneous analysis of amphetamines and their metabolic derivatives. In combination with LC–MS–MS, limits of quantitation (LOQ) of 0.54 ng/mL are achieved for MAMPH, HMMA, and MDMA and LOQ of 1.0 ng/mL are achieved for AMPH and MDA on Gemini C18 HPLC columns.
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
