The State of The Art in Oligonucleotide Analysis: An LCGC International Peer Exchange : Episode 10

Alternative Modes of Liquid Chromatography for Oligonucleotides

September 30, 2024

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This episode focuses on alternative modes of liquid chromatography (LC) for oligonucleotides as current go-to chromatographic modes may face significant changes in the future.

EP: 1.Introducing the Panel: The State of The Art in Oligonucleotide Analysis

EP: 2.Challenges in Analyzing Antisense Oligonucleotides (ASOs)

EP: 3.Chemistries and Products Available for ASOs

EP: 4.Discussing Analytical Strategies and 2DLC

EP: 5.The Regulatory Stance on Oligonucleotide Enantiomers

EP: 6.Future Perspectives and Fit-For-Purpose Separation Power

EP: 7.Robustness and Reproducibility: Is There a Competency Crisis?

EP: 8.On-Column Structural Formations

EP: 9.PFAS and Fluorinated Compounds: Considering Potential Future Restrictions

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EP: 10.Alternative Modes of Liquid Chromatography for Oligonucleotides

Historically, most products and methods are produced with similar modes of chromatography. For oligonucleotides, reversed-phase ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography (HILIC) are two common modes. Considering the potential of future restrictions, the panel talks about alternative modes of LC for oligonucleotides.