Multiplexed CE Analysis Sans Detector
Scientists at the National Institute of Standards and Technology (NIST, Gaithersburg, Maryland) have developed a multiplexed capillary electrophoresis system and technique for high-throughput separations.
Scientists at the National Institute of Standards and Technology (NIST, Gaithersburg, Maryland) have developed a multiplexed capillary electrophoresis system and technique for high-throughput separations. Their system consists of a capillary array with 3-mm-long capillaries that connect individual sample reservoirs to a common buffer reservoir. The technique uses an electric field and buffer counterflow to enable separations. They reduced the counterflow until each analyte was able to enter the channel, and the variation in current over time produced a set of simultaneous electropherograms. They expect the system to be suitable for scale-up to large numbers of simultaneous analyses. The current application is measurements of enzyme activity.
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
