HPLC 2017 Tuesday Morning Sessions
Session Title: Supercritical Fluid Chromatography (FUN 5)
Chairs: Davy Guillarme and Lucie Nováková
08:30 -Caroline West: Ions and SFC: An Improbable Match?
09:00 -Didier Thiébaut: Evaluation of Alternative Solvents in Normal Phase Liquid and Supercritical Fluid Chromatography for Lipid Classes Analysis
09:20 -Abhijit Tarafder: Modeling Retention Behavior Under Solvent Gradient Condition in SFC
09:40 -Charlene Muscat Galea: Optimization of Temperature and Back-Pressure in Drug-Impurity Profiling by SFC: A Response Surface Design Approach
Session Title: Electrodriven Separations–MS (HYP 5)
Chairs: Marja-Liisa Riekkola and Andras Guttman
08:30 -Jonathan Sweedler: Measuring Endogenous D-Amino Acids in the Brain - From Brain Regions and Individual Cells to Cellular Release - Using CE and LC
09:00 -Rawi Ramautar: Resolving Volume-Restricted Metabolomics Using Sheathless Capillary Electrophoresis-Mass Spectrometry
09:20 -Christian Neusüß: Mass Spectrometric Characterization of Impurities of an Antibody Separated by SDS-Capillary Sieving Electrophoresis Using CSE-CZE-MS
09:40 -Aran Paulus: Capillary Electrophoresis-Mass Spectrometry for Intact Mass Analysis of Antibodies and Antibody-Drug-Conjugates
Session Title: Chiral Separations (APP 5)
Chairs: Wolfgang Lindner and Alberto Cavazzini
08:30 -Bezhan Chankvetadze: Recent Trends in Application of Polysaccharide-Based Chiral Selectors for Liquid Phase Separation of Enantiomers
09:00 -Gerhard Scriba: Capillary Electrophoresis in the Determination of the Stereoisomeric Purity of Drugs
09:20 -Kenji Hamase: Three-Dimensional HPLC Analysis of Amino Acid Enantiomers in Complicated Real World Samples
09:40 -Tivadar Farkas: Further Studies on the HILIC Character of Polysaccharide-Based Chiral Stationary Phases in Polar Organic Separation Mode
Session Title: Metabolomics and Lipidomics (YOU 5)
Chairs: Thomas Hankemeier and Michael Witting
08:30 -Liang Li: High-Coverage Quantitative Metabolomics Using Chemical Isotope Labeling LC–MS
09:10 -Francesca Rigano: Use of a Novel Linear Retention Index System as Identification Tool in Liquid Chromatography: Applications in Lipidomics
09:22 -Evelyn Rampler: Simultaneous Non-Polar and Polar Lipid Analysis by Complementary Online Two-Dimensional Liquid Chromatography and High Resolution Mass Spectrometry
09:34 -Hanne Roberg-Larsen: Oxysterols in Cancer
09:46 -Tim Causon: Increasing Peak Capacity and Selectivity of LC–MS by Incorporation of Low-Field Drift-Tube Ion Mobility Separation
Session Title: Novel High-Efficient Separation Media (FUN 6)
Chairs: Andrew Alpert and Frank Steiner
10:30 -Mary Wirth: Protein HPLC with Polymer Bonded Phases on Silica
11:00 -Jelle De Vos: Microfluidic Chip Technology to Advance Multidimensional Liquid Chromatography
11:20 -Yi Chen: Long-Range Uniform Photonic Crystals for High Performance Separation
11:40 -Kenichi Nagase: Thermoresponsive-Ionic Block Copolymer Brush Modified Stationary Phase for Thermally-Modulated Proteins Separation
Session Title: Nanofluidic and Microfluidic Separations–MS (HYP 6)
Chairs: Robert T. Kennedy and Peter Willis
10:30 -Robert T. Kennedy: HPLC–MS Analysis of Small Samples at High Pressures
11:00 -Andrea Gargano: Online Nano 2DLC Meets Top-Down MS: WCX-HILIC/a×m/RPLC UVPD-HRMS Analysis of Histone Proteoforms
11:20 -Wim De Malsche: New Developments in Pillar Array Column Technology
11:40 -Rico Warias: Chip-Based Liquid Chromatography–Mass Spectrometry: An Integrated Tool to Study Catalysis at the Microscale
Session Title: Lipidomics (APP 6)
Chairs: Gerhard Liebisch and William Craig Byrdwell
10:30 -Markus R. Wenk: Natural Variation of Blood Plasma Lipids in Healthy Asian Individuals
11:00 -Huwei Liu: Lipidomic Analysis of Plasma in Patients with Lacunar Infarction Using Normal-Phase / Reversed-Phase 2D LC–MS/MS
11:20 -Miroslav Lísa: Lipidomic Analysis of Biological Samples: Comparison of Liquid Chromatography, Supercritical Fluid Chromatography and Direct Infusion Mass Spectrometry Methods
11:40 -Michael Witting: Ion Mobility as Additional Separation Dimension to Tackle the Complexity of the Caenorhabditis Elegans Lipidome
Session Title: Chiral and Achiral Stationary Phases (YOU 6)
Chairs: Bezhan Chankvetadze and Alla Chernobrovkina
10:30 -Wolfgang Lindner: Principles of Enantioseparations in LC: State of the Art, Quo Vadis
11:10 -Ravindra Hegade: Stationary Phase Optimized Selectivity Chiral Liquid Chromatography (SOS-CLC) as a Novel Perspective for the Separation of Stereoisomers
11:22 -Judyta Hejniak: Flavanones Enantioseparation by LC–ESI-MS/MS and its Application
11:34 -Magdalena Skoczylas: Multi-Parametric Characterization of Amino Acids- and Peptide-Silica Stationary Phases – A Column Selection for Separation Targets
11:46 -Romana Jarosova: Nitrogen-Incorporated Tetrahedral Amorphous Carbon Thin-Films – A New Electrode for the HPLC-EC of Biological and Environmental Analytes
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.