HPLC 2009 Show Dailies

Wednesday Aftrernoon

To begin the afternoon Robert Kennedy, Fritz Emi and Paola Dugo will present tutorials on Process Scale Batch and Continuous Chromatographic Separations, Quality by Design and Principles and Applications of Speciation Analysis.

The tutorials will then be followed by the afternoon’s sessions on Monoliths, Quality by Design and LC–MS and CE–MS by Frantisek Svek, Fritz Erni and Gerard Hopfgartner.

Nobua Tanaka discusses high efficiency monolithic silica capillary columns in the first lecture of the Monoliths sessions. Fabrice Gritti then discusses mass transfer in monolithic columns with different average pore sizes followed by a lecture by Karin Cabrera on the influence of the macro- and mesopore size on the performance of monolithic HPLC columns. Emily Hilder closes the session with a talk on improving the performance of polymer monoliths to separate small molecules.

The Quality by Design sessions begins with a keynote lecture on QbD and analytical methods for European companies by Mathias Pohl followed by a talk on the impact of the new ICH quality paradigm on the European Pharmacopoeia. Christoph Meyer gives his views on QbD for HPLC from the perspective of the pharmaceutical industry and Imre Molnar reveals his approach to QbD involving HPLC method development.

The session on LC–MS and CE–MS starts with Yehia Mechref on multiple reaction monitoring LC–MS for the monosacharride compositional analysis of glycoproteins followed by a lecture on ultrahigh-resolution MS in fast LC coupling for the analysis of complex mixtures. Pierangela Palma discusses matrix effects when using LC–MS with direct EI interface and Julian Schappler closes the session with a talk on blood doping with HBOC using CE-UV–ESI-TOF-MS.

Wednesday Morning

Welcome to Day Three of LC•GC Europe’s HPLC 2009 Show Daily.

The Wednesday morning parallel sessions kick off with a second round of seminars on Biopharmaeutical and Biomedical Analysis and Electroseparations chaired by Steve Cohen and Gerard Rozing and a session on Multidimensional Separations, chaired by Martin Gilar.

The first seminar in Multidimensional Separations section by Tyge Greibrokk assesses the advantages and limitations of multidimensional LC and is followed by Paola Dugo with a talk on different approaches to obtain high peak capacities in HPLC separations. Xiangmin Zhang describes his work on integrating multidimensional chromatography mass spectrometric methods for intact protein analysis and Sebastian Eeltnik reveals his findings on the design of monolithic columns for maximizing the LC performance in two-dimensional chromatography for the separation of complex proteomics samples in the concluding lecture.

The Biopharmaeutical and Biomedical Analysis section starts with a lecture by Barry Karger on the comprehensive characterization of protein targets and biopharamaceuticals using advanced LC–MS and CE–MS methods. Hanno Stutz then describes comprehensive characterization of diagnostic recombinant allergen products with complementary CE strategies and Frank Sinner will describe the role of ring-opening metathesis polymerization-derived capillary monoliths in modern biomedical research. Egidijus Machtejevas concludes with an overview of 2D LC for the analysis of complex peptide samples.

Capillary electrochromatography and molecular dynamics simulations in human nanoscale interaction studies is discussed by Marja-Lisa Riekkola in the first Electroseparations session, followed by a talk by H. Watzig on precision in capillary isoelelectric focusing for long-term protein analysis. Markus Martin will discuss using CE–ESI-MS–MS as a rapid screening tool for determining protein–ligand interactions and binding constants and David Goodall describes selective focusing of charged analytes in electrically-assisted LC on monolithic media.

The next round of parallel sessions on Wednesday on Monoliths, Proteomics and Pharmaceutical Analysis are chaired by Nobuo Tanaka, Peter Oefner and Pat Sandra respectively.

Frantisek Svec kicks off the session on Monoliths with a lecture on the art of pore surface functionalization to prepare monoliths tailored for specific applications, followed by Michael Buchmeiser on electron-beam curing and ROMP-derived monolithic devices. The purification of biomacromolecules using methacrylate monoliths is described by Ales Podgornik and Andreas Greiderer assesses the influence of polymerization time on porous properties of organic monolithic stationary phases.

Part two of the Proteomics sessions begins with a talk by Fred Regnier engagingly titled Humans are More Stressed Than We Realize: Oxidation of Proteins and is followed by Alan Van Dorsselaer on genome annotation and TMPP derivitization. Oliver Kohlbacher discusses how to make the most of your data in high throughput proteomics data. This section ends with a lecture on the development of a high resolution MudPit method for the identification of neuroproteins in mice by Karl Mechtler

The Pharmaceutical Analysis sessions begin with a talk on the separation of pharmaceutical compounds at high temperatures using HPLC by Roger Smith, followed by Xiaoli Wang on the critical comparison of sub-2 µm particles and superficially porous particles under optimized ultra-high pressure conditions in pharmaceutical analysis. Caterina Temporini describes the use of frontal affinity chromatography with MS to identify potential drug candidates and Pawl Wiczling discusses the role of gradient reversed-phase HPLC to lipophilicity and the pKa determination of drugs.

Poster sessions and vendor seminars take place during the lunchbreak.

Tuesday Afternoon

Bruno Bolinger, Guenther Bonn and Rainer Bischoff will present tutorials on Validating Analytical Methods, Modern Stationary Phase Technologies and Biomarker Discovery and Validation.

The Tuesday afternoon sessions chaired by Michael Lammerhoffer, Guenther Bonn and Marja Lisa Riekkola on Column Technology, Industrial Analysis and Biopharmaceutical and Biomedical Analysis will then begin.

Uwe Neue describes a new world of UHPLC and HILIC application in the first Column Technology lecture. This will be followed by Judi Abia on radial heterogeneity of some analytical columns used in HPLC. Andrew Shalliker will discuss the importance of column bed heterogeneity and viscosity contrast for solute elution profiles. Christian Huck will close this session by describing the importance of the simultaneous determination of physical and chemical parameters of porous polymers with a combined use of Fourier-Transform near infrared diffuse reflection spectroscopy with multivariate techniques.

The Industrial Analysis lectures comprise John Frenz on the challenges in the manufacture and characterization of inactivated yeast therapeutic vaccines, Franka Kalman on the use of molecular CE to develop robust and reliable industrial biopharmaceutical test methods, Dell Farnan on multi-product charge heterogeneity profiling of recombinant monoclonal antibodies using PH gradient ion exchange chromatography and G. Choudray on the analytical characterization of inhalable powders.

The Biopharmaceutical and Biomedical Analysis session starts with Rui Zhao on the characterization of human plasma intact peptides and LMW proteins and their modifications using HELC–FT-MS–MS–UStags, followed by Rayane Mohamed on a comprehensive analytical strategy for the structural identification of biomarkers in biofluids. Melina Quaglia will describe work on LC–MS for absolute protein quantification and Djuro Josic closes with a seminar on proteomics as a tool for the optimization of human plasma-derived therapeutic proteins.

Tuesday Morning

Welcome to Day Two of LC•GC Europe’s HPLC 2009 Show Daily.

The Tuesday morning programme of HPLC 2009 begins with parallel sessions on Proteomics, Molecular Basis of Separations and Environmental Analysis chaired by Bill Hancock, Attila Felinger and Guowang Xu respectively.

The Proteomics slot starts with a seminar on a micro-proteomics study of genetically engineered mouse models by David Lubman followed by a discussion of novel separation and identification techniques in proteomics by Yukui Zhang. The application of Displacement Chromatography for bottom-up and top-down proteomics approaches is covered by Helmut Schluter and the session closes with a talk by Edward Nice on the potential of faecal proteomics to detect and monitor colorectal cancer.

The Environmental Analysis lectures start with a talk by Thomas Ternes on LC quadrupole–linear ion trap MS to identify polar transformation products from Iodinated X-Ray contrast media followed by a case study by Guibin Jiang on a novel method to detect a brominated flame retardent near a manufacturing plant in China. Jin-Ming Lin describes a novel method of analysing phenolic compounds in environmental water samples using molecularly imprinted micro-solid-phase extraction combined with HPLC. Jun Haginake closes this session with an illuminating talk on a highly selective method to analysis of non-steroidal anti-inflammatory drugs in water samples using restricted access media-molecularly imprinted polymers.

Klaus Albert discusses ways to improve the understanding of the properties and retention behaviour of chemically bonded stationary phases in the keynote lecture for the Molecular Basis of Separations slot. This is followed by a discussion on a new approach on the solvation processes in reversed-phase liquid chromatography by Boguslaw Buszweski and a talk on comparing immunosorbents and MIPs for the selective extraction of a target analyte from complex samples by SPE sorbents based on aptamers. The closing lecture in this session by Sergey Sergayev describes enantioseparation of symmetrical and non-symmetrical analogues of Trogers base.

The second round of parallel sessions on Tuesday morning are Multidimensional Separations and Column Coupling, chaired by Peter Schoenmakers, Metabolomics, with Katja Detttmer and Chiral and Food Analysis chaired by Vadim Davankov.

After a coffee break the Multidimensional Separations sessions begin. First is a talk on the optimization of two-dimensional HPLC by Georges Guichon, followed by a discussion by Frank Steiner on the optimum LC set-up to separate all peaks in the shortest possible time. Pavel Jandera discusses the perspectives and limitations of reversed-phase HILIC comprehensive LC×LC and Deirdrie Cabooter describes the use of the kinetic plot method to design single and coupled column systems.

Ian Wilson starts the Metabolomics section with a stimulating lecture on the role of hyphenation in metabolic profiling. The challenges of using LC–MS metabonomics to diagnose disease and predict disease development are discussed by Guowong Xu and Multi-target Quantitative Metabolic Profiling by HPLC–ESI-MS–MS is covered by Michael Lammerhofer. Theresa Koal closes the session with a talk on MS–MS-based targeted metabolomics in biological samples with — and without — chromatography.

The Chiral and Food Analysis lectures includes Vadim Davankov on the dual separation mechanism of enantiomers in capillary electrophoresis, Thomas Hoffman on tracing the taste compounds in foods by combining chromatography and analytical sensory tools, Pilar Franco on finding the best separation for enantiomeric mixtures and Melanie Vogel on the determination of pesticides in different food matrices using LC–MS–MS.

Poster sessions and vendor seminars are showcased during the lunchbreak.

Monday Afternoon

Monday’s afternoon sessions will kick off with David McCalley, Karl-Siegfried Boos and Stephan Lamotte, as well as D. Belder delivering parallel troubleshooting tutorials on how to obtain good peak shapes and efficiencies when using reversed phase and hydrophilic interaction separations, how to overcome problems in bioanalytical LC–MS–MS and how to overcome obstacles related to the miniaturization of separation processes respectively.

Heinz Engelhart, Karl-Siegfried Boos and Frank Steiner then chair parallel sessions on the work of Barry Karger, Clinical Analysis and a second series of lectures on Fundamental Aspects of HPLC.

Highlights of the Barry Karger sessions include Wolfgang Lindner on the hope (or hype) attached to HILIC-type selectivities, Frantisek Foret on the potential of nanotechnology in bioanalysis and Andras Guttman on new developments in fluorescent isotope coded affinity tag (FCAT) labelling of tryptic peptides. Wolfgang Gotzinger closes the event with his findings on achiral purification of small molecules by SFC and role of MS-directed purification.

Topics in the Clinical Analysis session include selective ESI-MS pre-column derivitization reagents for screening aldehyde biomarkers by Hubertus Irth, Molarino on assessing renal function for potential kidney donation using iothalamate quantification using UPLC–MS–MS, Ron Majors on trends in sample prep and Rosa Morello on in-line processing of whole blood using SPE-LC–MS–MS.

The second part of the Fundamental Aspects series kicks off with Gert Desmet critically appraising Giddings and general rate plate height models, Torgny Fornsted on accurate in-depth characterization of analytical and preparative phase systems, Kanji Miyabe will discuss a numerical method to analyse tailing peak profiles and A. de Villiers will discuss the effect of analyte properties on the kinetic performance of LC separations.

Monday Morning

HPLC 2009 begins today in earnest with a plenary lecture from Hans Maurer on current trends in LC-MS in clinical and forensic science followed by a review of current applications and future challenges of HPLC-MS in drugs and metabolites analysis by Gerard Hopfgartner.

Andreas Manz chairs the Microscale Analysis session, which includes lectures from Gunther Fuhr on single cell separation and manipulation, Salvatore Fanali on nano-liquid chromatography in food and drug analysis, Jeremy Galineau on the future of microscale analysis and the session closes with a talk on miniaturized on-line analysis of a target protein in plasma samples by Annabelle Cingoz.

The Fundamental Aspects chaired by Georges Guiochon will start with a talk by Attilla Felinger on mass transfer in liquid chromatography followed by Fernando Lancas on temperature-programmed capillary liquid chromatography, Ulrich Tallarek on transport phenomena in packed microchips; and closes with a talk by Jan Stahlberg on the circumstances when the ideal model of gradient chromatography actually applies.

Edward Yeung chairs the Electroseparation session, which will include lectures on inorganic explosive identification using capillary electrophoresis and ion chromatography by Paul Haddad, Gerard Rozing discussing "capillary electrophoresis rennaisance" and new applications of the technique, Christian Klampfi on the future role of ESI and Non-ESI in CE–MS and will close with a session on electromembrane extraction in microscale sample preparation by Astrid Gjelstad.

Poster sessions and vendor seminars take place during the lunchbreak.