Flash Chromatography

Flash chromatography is a type of preparative liquid chromatography commonly used in the separation of organic compounds. It utilizes a plastic column filled with some form of solid support, usually silica gel, with the sample to be separated placed on top of this support. The rest of the column is filled with an isocratic or gradient solvent that with the help of pressure, enables the sample to run through the column and become separated.

2009 flash demand by industry.

Flash chromatography has become a popular method of normal phase separation through purification. While flash chromatography is typically a low-pressure technique, scientists are using vacuums or pumps at medium pressures to speed up the separation process. The columns are packed with a silica adsorbent of defined particle size usually between 30–60 μm, although other packings with other particle sizes are also used. Mobile phases with low viscosity require smaller particle sizes.

Demand for flash chromatography comes primarily from the pharmaceutical and biotech industries accounting for nearly three-fourths of the market. The technique is commonly used in the separation of synthetic organic compounds, which are particularly useful in drug discovery. Flash chromatography systems assist laboratories focusing on combinatorial chemistry to streamline their purification steps.

The foregoing data was extracted from SDi's Global Assessment Report, 11th Edition. For more information, contact Glenn Cudiamat, VP of Research Services, Strategic Directions International, Inc., 6242 Westchester Parkway, Suite 100, Los Angeles, CA 90045, tel. (310) 641-4982, fax (310) 641-8851, e-mail:cudiamat@strategic-directions.com