Detecting Nitric Oxide with Capillary Electrophoresis
Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells.
Researchers from the Second Military Medical University (Shanghai, China) used capillary electrophoresis with laser-induced fluorescence detection to detect the release of nitric oxide from single neuron and mammalian cells. They used 8-(3,4-diaminophenyl)2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene as the fluorescent probe. They studied the feasibility of applying the method to four single-cell models. The method could be a powerful tool for studying the kinetics of nitric oxide release by neuronal cells during neurotransmission and for examining the pathobiological and therapeutic basis of nitric oxide for cardiovascular diseases.
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
