An Analysis of Amino Acids in Oxidized and Unoxidized Feed Samples
Commission Regulation (EC) No 152-2009, published in TheOfficial Journal of European Union, laid down the methods of sampling and analysis for the official control of feed. The regulation describes methods of analysis to control the composition of feed materials and compound feed products. Establishing the amino acids profile is an important way to control quality and nutritional value of feeds. This regulation specifies HPLC with post-column derivatization with ninhydrin reagent as the method of analysis for total and free amino acids. Pickering Laboratories developed an analytical method to comply with all the chromatographic requirements of Commission Regulation (EC) No 152-2009. The same method is used to analyze oxidized and unoxidized feed samples.
Figure 1: Chromatogram of a standard solution of amino acids
Figure 2: Chromatogram of an oxidized feed sample
Figure 3: Chromatogram of a non-oxidized feed sample
Method
Table I: HPLC Program
Table 2: Column oven program
Pickering Laboratories
1280 Space Park Way, Mountain View, CA 94043
tel. (800) 654-3330, (650) 694-6700, fax: (650) 968-0749
Website: www.pickeringlabs.com
Analytical Challenges in Measuring Migration from Food Contact Materials
November 2nd 2015Food contact materials contain low molecular weight additives and processing aids which can migrate into foods leading to trace levels of contamination. Food safety is ensured through regulations, comprising compositional controls and migration limits, which present a significant analytical challenge to the food industry to ensure compliance and demonstrate due diligence. Of the various analytical approaches, LC-MS/MS has proved to be an essential tool in monitoring migration of target compounds into foods, and more sophisticated approaches such as LC-high resolution MS (Orbitrap) are being increasingly used for untargeted analysis to monitor non-intentionally added substances. This podcast will provide an overview to this area, illustrated with various applications showing current approaches being employed.
RAFA 2024 Highlights: Cutting-Edge Chromatography Techniques for Food Safety and Food Analysis
November 18th 2024An illuminating session focusing on progress in analytical techniques used in food analysis took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, The Czech Republic, including a talk on the analysis of 1000 toxins in 10 minutes.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
Profiling Volatile Organic Compounds in Whisky with GC×GC–MS
November 1st 2024Researchers from Austria, Greece, and Italy conducted a study to analyze volatile organic compounds (VOCs) present in Irish and Scotch whiskys using solid-phase microextraction (SPME) Arrow with comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GC×GC–MS) to examine the organoleptic characteristics that influence the taste of spirits.
