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Effects of Buffer Capacity in LC, Part II: Visualization of pH Changes Inside the Column
February 1st 2020In situ measurements of the mobile-phase pH before and after the column help to rationalize the effects of mismatch in pH and concentration between the mobile phase and sample buffer mismatch in reversed-phase LC separations.
State-of-the-Art in Capillary Liquid Chromatography (LC): Now, Next, and How?
At the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, a panel addressed the current challenges and potential future directions in capillary liquid chromatography. How can their vision be achieved?
State-of-the-Art in Capillary Liquid Chromatography: Now, Next, and How?
The “State-of-the-Art in Capillary Liquid Chromatography” panel discussion at the 43rd International Symposium on Capillary Chromatography (ISCC 2019) in Fort Worth, Texas, USA, was a thoughtful dialogue on current challenges and potential future directions in the field. The session included a general overview of the current state of the field, key drawbacks preventing widespread use of capillary liquid chromatography (LC) columns, and how these challenges might be overcome. In this article, we highlight some of the common themes that were discussed as part of this panel.
The LCGC Blog: Climbing the Academic Career Ladder: Choices at the Top
January 7th 2020Looking back, the start of my penning of blog articles matches quite well with the start of efforts to investigate the potential environmental impacts of unconventional oil and gas extraction, and the formation of the Collaborative Laboratories for Environmental Analysis and Remediation (CLEAR; http://clear.uta.edu) at the University of Texas Arlington. It also well coincided with my effort to begin some outside consulting activities, predominantly the review of forensics evidence for blood alcohol determination. Both of these activities have blossomed considerably in the past seven years, and it has become interesting to contemplate the most rewarding way to spend my time going forward.
Thirty-Two Years Connecting with Gas Chromatography
January 1st 2020I have edited the “GC Connections” column in LCGC for over 32 years. In that time, gas chromatography (GC) has changed from a specialized separations technique to being more routine for standardized methods, while expanding outward to complex multidimensional separations and scaling downward towards miniaturization and portability. In my last “GC Connections” article before stepping away from the role of Editor, I give some perspective on how GC has changed and grown over the years, and where it might go in the future.
Making the Most of Size‑Exclusion Bioseparations Using Sub‑2‑µm Column Technology
January 1st 2020Size-exclusion chromatography (SEC) is a mainstay in the biopharmaceutical industry, serving as a gold standard analytical tool for the characterization of therapeutic proteins in development and manufacturing settings. Contemporary SEC separations can be performed using columns packed with sub-2-μm particles, and these platforms offer the highest efficiencies available for the separation of monoclonal antibody monomer species from low- and high-molecular-weight product-related impurities. Compared to other chromatographic modes used to characterize proteins, SEC is unique in that analytes are not retained by the stationary phase. As a result, special care is required to achieve in practice the chromatographic efficiency that is expected in theory. In this article, we describe the fundamental aspects of achieving high performance using sub-2-μm SEC columns. In addition, we discuss trends in the biopharmaceutical industry, including challenges that can be addressed using modern size-exclusion technologies.
