Application Notes: LC-MS

In reversed-phase liquid chromatography, increasing the column temperature usually results in a decrease in analyte retention. Will this cause problems if a method does not call for control of the column temperature but the room temperature of the laboratory environment changes?

HCPs can threaten drug product efficacy and stability. LC–MS/MS using automated sample prep can address HCP analysis challenges. Software and hardware workflow upgrades can optimize HCP analysis methods.

This application demonstrates the efficacy of this chromatographic technique for a fast orthogonal analysis to evaluate mAb ADCC activity.

Learn how Janssen cut method-development time by 80% while improving quality 25-fold by upgrading their hardware and using method-development software.

A simple and sensitive method is presented for the direct analysis of Glyphosate and AMPA in drinking water without sample derivatization or pre-concentration.

This RAFA 2019 winner of the Best Poster Award, reviews the identification and characterization of secoiridoids isomers found in Greek extra virgin olive oil (EVOO).

Automated GC/MS determination of 3-MCPD and glycidol in edible oils. Evaporation step helps reach required LODs with standard MSD and removes excess derivatization reagent for improved stability.

Solid-phase extraction (SPE) is a powerful technique for LC­–MS analysis. SPE offers benefits for various solutes from a wide range of samples. Modern SPE systems can remove unwanted compounds and streamline methods.

This application note showcases a fast and robust solution for analysis of all analytes listed in EPA Method 8327, showing reliable, sensitive quantitative results.

A fast and reliable method for comparison of related impurities in a brand and a generic over-the-counter (OTC) acetaminophen drug.

- ADCs can be analyzed using various methods other than the traditional HIC. - New chromatographic materials expand analytical techniques for APOC

Here, we demonstrate increased quantitative performance and throughput efficiency on a Thermo Scientific™ Orbitrap Exploris™ 480 LCMS combined with a FAIMS Pro™ device.

Here we investigate the optimal isolation width for the ID and quantification of proteins and peptides for the best possible accuracy and precision in LFQ experiments.

Here we describe an internal standard (IS)-guided LC-MS acquisition workflow for reproducible, sensitive and high-density absolute quantification of pTyr signaling.

Here we discuss the benefits of Real Time Search & advanced spectral processing algorithms on an Orbitrap Tribrid MS to enhance TMT quan accuracy and proteome coverage.

With Antec Scientific’s new µ-PrepCell-SS, electrochemical reduction of disulfide bonds in proteins, mAbs, and other biopharmaceuticals is possible in any LC-MS workflow.

With Antec Scientific’s new µ-PrepCell-SS, electrochemical reduction of disulfide bonds in proteins, mAbs, and other biopharmaceuticals is possible in any LC-MS workflow.

µPAC™ technology has been used for analysing HCP impurities originating from recombinant expression systems used in the production of mAb based biotherapeutics.

Testing shows that Restek Long-Life ESI electrodes for Sciex Turbo V sources provide equivalent chromatographic performance to original equipment manufacturer electrodes over significantly longer lifetimes.

FDA recently posted an LC–HRMS method using the novel ACE3 C18-AR column, which can be used by regulators to detect “probable human carcinogen” NDMA in ranitidine.

An SFC-MS/MS method for high-sensitivity analysis of 34 PAs in commercial tea samples was developed.

• Analyzing biogenic amines in food by adding ion-paring agents • Case studies on polar aminoglycosides; paraquat/diquat; and BAs in food

Automated metabolic stability assays with sample incubation and LC-MS/MS determination are presented for use in drug metabolism and pharmacokinetic (DMPK) laboratories.

Fully automated extraction, removal of matrix interferences (phospholipids), and accurate determination of antibiotic compounds in eggs by LC-MS/MS is demonstrated.

Learn more on automating the isolation of PFCs from tap water prior to their analysis by LC/MS systems, with results showing good reproducibility and reduced human labor time.

Learn more on automating the isolation of PFCs from tap water prior to their analysis by LC/MS systems, with results showing good reproducibility and reduced human labor time.

Strata-X PRO was chosen to extract a panel of acids, neutrals, and bases from plasma. This simple method eliminates time-consuming method development and recovery for extremely polar analytes.

Using Strata-X PRO to extract barbiturates from serum saves time by reducing the method to 3 quick and easy steps. In addition, the matrix is more effectively clean-up vs protein precipitation.