Oligonucleotide Analysis by Ion-Pair Chromatography

Crucial phosphorylated compounds can be analyzed by a polymeric iHILIC-(P) Classic HILIC column and MS. The method is applicable for targeted and non-targeted metabolomics.

This non-IP-RP method, using ammonium bicarbonate as mobile phase additive, is highly sensitive and suitable for the bioanalysis of therapeutic oligonucleotides.

The optimized LC-MS/MS method covering 388 signaling lipids shows that the bioinert coated YMC Accura Triart C18 column combines high sensitivity with high resolution.

In this application note, the charge variant characterisation of IgG4-based mAbs was performed using a BioPro IEX QF anion exchange column coupled to an MS detector.

The non-targeted screening of polar metabolites in human plasma with high sensitivity is achieved using a bioinert YMC Accura Triart Diol HILIC column.

The analysis of human plasma samples using a bioinert coated YMC Accura Triart C8 column demonstrates that real-life lipidomic analysis in under 10 minutes is possible.