Application Notes: LC-MS

For this peptide application, two different types of polymeric SPE (WCX and SAX) were tested in parallel with Strata-X PRO SPE and compared in regards to recovery and % variation.

Using Strata-X PRO to extract barbiturates from serum saves time by reducing the method to 3 quick and easy steps. In addition, the matrix is more effectively clean-up vs protein precipitation.

• Modern software makes tracking and scheduling maintenance more efficient • Systems schedule maintenance based on instrument usage

Dual GC poster that includes gas management selection chart and GC column selection chart

Zebron gas management filters and click-on GC traps remove contaminants from mobile-phase gases, reduce column bleed, increase column life, protect GC detectors.

Zebron gas management filter selection chart by the GC instrument and the fitting size

Zebron gas management filter selection chart by the GC instrument and the fitting size

Dual GC poster that includes gas management selection chart and GC column selection chart

Zebron gas management filters and click-on GC traps remove contaminants from mobile-phase gases, reduce column bleed, increase column life, protect GC detectors.

Zebron click-on trap step-by-step installation instruction

Zebron gas filter step-by-step installation instruction

Zebron electronic indicator technical information and installation steps

Zebron LC–MS gas filter step-by-step installation instruction

Zebron electronic indicator technical information and installation steps

Zebron click-on trap step-by-step installation instruction

Zebron Electronic Indicator technical information and installation steps

Zebron electronic indicator technical information and installation steps

Zebron gas filter step-by-step installation instruction

Zebron electronic indicator technical information and installation steps

Zebron Electronic Indicator technical information and installation steps

Zebron LC–MS gas filter step-by-step installation instruction

Solvent-reduced determination of 100 contaminants from low double-digit to low triple-digit pg/L in 100 mL surface water samples, meeting the requirements of the EU-WFD based on SBSE-TD-GC-MS/MS

Quantifies affinity, stoichiometry of biomolecular assemblies, helping understand the mechanism of interaction and develop therapeutics that target and modulate them.

Quantifies affinity, stoichiometry of biomolecular assemblies, helping understand the mechanism of interaction and develop therapeutics that target and modulate them.

A rapid, accurate, and reproducible method was developed for high-throughput testing of nicotine, cotinine, trans-3’-hydroxycotinine, nornicotine, norcotinine, and anabasine in urine. Data show that a fast and highly efficient analysis of these basic compounds can be achieved with the Raptor Biphenyl column using standard reversed-phase LC–MS mobile phases that are compatible with a variety of LC–MS instrumentation.

The term ionic liquid refers to organic salts with relatively low melting points (below 100 °C) that usually consist of an organic cation or anion and a counterion, in either organic or inorganic form. Ionic liquids exhibit unique characteristics such as extremely low vapour pressure, excellent thermal stability, electrical conductivity, a high degree of polarity and miscibility with various types of solvents. Ionic liquids have been used as catalysts and solvents in organic chemistry and electrochemistry and as mobile phase modifiers or functionalized stationary phases in separation science.1–3

Catecholamines are important markers for the diagnosis and management of tumour diseases of the sympathoadrenal system. The major catecholamines are dopamine, norepinephrine and epinephrine. Urine tests have shown to be applicable to measure the level of catecholamines in the human body. Various separation methods have been used for the clean-up of catecholamines in biological fluids: solvent extraction, adsorption on alumina, ion-exchange and solid-phase extraction of a diphenylboronic acid-catecholamine complex. From the point of simplicity, reproducibility and automation the last method is the most suitable and can be realized by on-line SPE–LC. Diphenylborate forms a negatively charged complex with the diol groups of catecholamines. These complexes which are strongly retained on a polystyrene-divinylbenzene cartridge in alkaline medium (pH 8.5) can be eluted in a second step from the SPE unit directly onto the HPLC column. The separation of catecholamines is performed in isocratic mode by..

This article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyse potentially harmful compounds.

Zhe Yin, Kenneth J. Fountain, Erin E. Chambers and Diane M. Diehl, Waters Corporation, Milford, Massachusetts, USA.

Based on the QuEChERS AOAC Official Method 2007.01, 16 pesticides were used for evaluating the performance of the Agilent's AOAC buffered extraction and SampliQ QuEChERS dispersive SPE kits for general fruits and vegetables.