Simultaneous Extraction and Rapid Analysis by LC–MS–MS of Melamine and Cyanuric Acid
Using solid-phase extraction sorbent followed by LC–MS–MS analysis using HILIC to measure melamine and cyanuric acid in under 3 mins.
The presence of both melamine and cyanuric acid in concentrations greater than 2 µg/mL results in the crystallization of the two compounds, forming the toxic, poorly water soluble melamine cyanurate complex. Increasingly, governing and regulatory bodies are requiring the testing of both melamine and cyanuric acid in processed food products. Many methods describing the simultaneous analysis of melamine and cyanuric acid have been published, however, to extract both compounds from food samples with the currently accepted method, analysts have to perform two solid-phase extraction procedures using a cation-exchange material and an anion-exchange material, for melamine and cyanuric acid respectively. This article describes a method for the simultaneous extraction of both compounds using one solid-phase extraction sorbent, Strata Melamine, followed by LC–MS–MS analysis using Luna HILIC to accurately measure melamine and cyanuric acid in less than 3 minutes.
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
