Protein Standards Increase HPLC Accuracy
A newly launched library of more than 5,000 human proteins is desinged for increased accuracy in high performance liquid chromatography and mass spectrometry analyses, according to life sciences company OriGene Technologies.
A newly launched library of more than 5,000 human proteins is desinged for increased accuracy in high performance liquid chromatography and mass spectrometry analyses, according to life sciences company OriGene Technologies.
The company has released a library of heavy isotope labelled human proteins to serve as international standards for mass spectrometry analyses featuring single reaction and multiple reaction monitoring (MRM). According to the company, the aim is to produce research tools that allow for disease mechanisms to be researched and drugs to be developed based across the entire human genome, with stem cell research and cancer as two areas of particular interest.
“Mass spectrometry - especially MRM - has emerged as a new platform for quantitative characterisation of multiple proteins, thus making it an ideal choice for high-throughput proteomics and clinical biomarker research,” the company stated in a press release.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
LC/MS Based Characterization Workflow of GLP-1 Therapeutic Peptide Liraglutide and Its Impurities
January 14th 2025This application note demonstrates a liquid chromatography/quadrupole time-of-flight mass spectrometer (LC/Q-TOF MS) workflow for the identification and characterization of therapeutic peptides.
