HPLC 2017 Monday Morning Sessions
Session Title: Characterization of HPLC Stationary Phases - In Memory of Jack Kirkland (FUN 1)
Chairs: Barry L. Karger and Joe DeStefano
08:30 -Attila Felinger: Mass Transfer Properties of Zwitterionic Chiral Stationary Phases
09:00 -Barry Boyes: Improving Biomolecule Separations with Superficially Porous Silica Particles
09:20 -Joseph Glajch: Characterization of HPLC Stationary Phases: How This Can Lead to Novel and Improved Chromatography
09:40 -Martin Gilar: Experimental Evaluation of Microfluidic LC Column Performance: Straight Versus Serpentine Channels
Session Title: Ion Mobility in LC–MS (HYP 1)
Chairs: Alexander Makarov and Karel Lemr
08:30 -Gérard Hopfgartner: Differential Mobility Spectrometry - Mass Spectrometry as an Orthogonal Separation Dimension for Omics
09:00 -Oliver Schmitz: LC+LC- and GC+GC-IM-qTOF-MS as a Potential Tool in Non-Target Analysis
09:20 -Alexey Makarov: Using IMS-MS Combined with Size-Exclusion Chromatography and Differential Hydrogen–Deuterium Exchange for Semi-Automated Screen of Global Protein Conformational Changes in Solution
09:40 -Qiang Ma: Comprehensive Separation and Analysis of Nonionic Surfactants by Ultra-High Performance Supercritical Fluid Chromatography Combined with Ion Mobility Spectrometry-Mass Spectrometry
Session Title: Forensics, Doping, and Toxicology (APP 1)
Chairs: Gerhard Scriba and Christian Klampfl
08:30 -Mario Thevis: Testing Non-Approved Substances Using Alternative Matrices and Liquid Chromatography / High- and Low-Resolution Tandem Mass Spectrometry in Doping Controls
09:00 -Andreas Thomas: Doping Control Analysis of Prohibited Peptide Drugs After Sophisticated Metabolism Studies by Means of Liquid Chromatography Coupled to HR-MS/MS
09:20 -Federico Ponzetto: Exploring Blood Steroidomics for the Improvement of the Athlete Biological Passport Steroidal Module
09:40 -Marieke De Boeck: Validation of a Fast Ionic Liquid-Based Dispersive Liquid-Liquid Microextraction Procedure Combined with LC–MS/MS Analysis for the Quantiï¬cation of Benzodiazepines in Whole Blood
Session Title: 2D-LC (YOU 1)
Chairs: Paula Dugo and Bob Pirok
08:30 -Mark Schure: Two-Dimensional Chromatography: A Tutorial
09:10 -Renata Gerhardt: Chip-Based HPLC with an Integrated Droplet Interface for Surface Enhanced Raman Detection
09:22 -Elisenda Fornells: Membrane Evaporative Interface for LCxLC
09:34 -Nikoline Juul Nielsen: Increasing Flexibility in Two-Dimensional Liquid Chromatography by Pulsed Elution of the First Dimension (Pulsed-Elution 2D-LC)
09:46 -Douglas Richardson: Two-Dimentional LC for Analysis of Monoclonal Antibody During Bioprocess Development
Session Title: Multi-Dimensional Separations (FUN 2)
Chairs: Kelly Zhang and Dwight Stoll
10:30 -Peter J. Schoenmakers: Multi-Dimensional Liquid Chromatography – Towards a Million Peaks
11:00 -Mark Schure: Size Exclusion Chromatography with Superficially Porous Particles
11:20 -Bob Pirok: Extended Multi-Dimensional Liquid-Chromatography Systems for Comprehensive Analysis of Complex Polymeric Nanoparticles
11:40 -Andre de Villiers: Pareto-Optimisation of HILIC×RP-LC Separations: Experimental Verification and Application to Phenolic Analysis
Session Title: Ion Suppression and Matrix-Effects in Separation – MS (HYP 2)
Chairs: Mario Thevis and Oliver Schmitz
10:30 -Jean-Luc Veuthey: Evaluation of Matrix Effects in HILIC, RPLC and SFC-MS
11:00 -Lucie Nováková: Development of UHPSFC-MS Method for High-Throughput Analysis of Vitamin E Isomers and Evaluation of Matrix Effects When Using Different Sample Preparation Approaches
11:20 -Aimin Tan: How Much Separation for LC–MS/MS Quantitative Bioanalysis?
11:40 -German Gomez-Rios: Can HPLC-MS be Replaced by Direct Coupling of SPME to MS? Investigating Challenging Cases
Session Title: Proteomics (APP 2)
Chairs: Sebastiaan Eeltink and Ying Ge
10:30 -John Yates: Protein-Protein Interaction Studies of Disease Networks
11:00 -Christian G. Huber: Proteome- and Phosphoproteome Analysis of Onkogenic Signaling Pathways
11:20 -Marcel Kwiatkowski: Better Access to Proteoforms by Cold Vaporization of Tissues with Picosecond-Infrared-Laser (PIRL) Ablation
11:40 -Morteza Khaledi: Surfactant-Free Coacervation of Natural Cell Membranes for Facile, Selective Extraction, and Enrichment of Integral Membrane Proteins in Proteomics
Session Title: Separation Mechanisms (YOU 2)
Chairs: Martin Gilar and Terence Hetzel
10:30 -Andrew Alpert: Effect of Salts on Retention of Neutral Solutes in HILIC
11:10 -Sander Deridder: Numerical Study of the Thermal Conductivity of Columns Packed with Fully Porous and Superficially Porous Particles
11:22 -Katarzyna KrzemiÅska: Dual Retention Mechanism on Stationary Phases with Embedded Polar Group
11:34 -Khac-Long Nguyen: Transport of Molecules of Different Sizes Through Monolithic, Core-Shell and Porous Silica Columns. Effect of Porous Silica Architecture
11:46 -Szymon Bocian: Solvation Processes in Liquid Chromatography - The Importance and Measurement
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.