HPLC 2017 Monday Afternoon Sessions
Session Title: Mechanisms of Mass Transport Phenomena (FUN 3)
Chairs: Pavel Jandera and Frederic Lynen
14:00 - Fabrice Gritti: Combining Solvent and Non-Uniform Temperature Gradients to Improve Peak Capacity in Microfluidic Separations
14:30 - Deirdre Cabooter: Experimental Methodologies for the Assessment of Individual Mass Transfer Phenomena Revisited
14:50 - Szabolcs Fekete: System Band Broadening and its Impact in Modern Size-Exclusion Chromatography of Proteins
15:10 - Julia Rybka: Surface Diffusion of Aromatic Hydrocarbon Analytes in Reversed-Phase Liquid Chromatography
Session Title: Separation – High-Resolution MS (HYP 3)
Chairs: Michal HolÄapek and Zoltán Takáts
14:00 - Alexander Makarov: Orbitrap Technology in LC/MS and GC/MS: On the Road to High-Resolution in Every Lab
14:30 - Ying Ge: Top–Down Proteomics of Large Proteins up to 223 kda Enabled by Serial Size Exclusion Chromatography Strategy
14:50 - Govert Somsen: Highly Selective Characterization of Intact Proteoforms by HILIC-MS
15:10 - Christian Lanshoeft: A Generic Hybrid LBA-LC-HRMS-Based Workflow for Multiplexed hIgG1 Quantification in Pre-Clinical Species Directly at the Intact Protein Level
Session Title: Sample Preparation and Automation (APP 3)
Chairs: Emily Hilder and Doo Soo Chung
14:00 - Janusz Pawliszyn: Why are Thin Coated Sampling/Extraction Devices with Biocompatible Sorbents Well Suited for Coupling of Biological Systems to Liquid Chromatography/Mass Spectrometry?
14:30 - Gongke Li: Novel Online Sample Preparation Media for Complicated Samples Analysis Coupling with High-Performance Liquid Chromatography
14:50 - Massimo Morbidelli: Isoform Enrichment by Recycling Chromatography
15:10 - Astrid Gjelstad: Does Parallel Artificial Liquid Membrane Extraction Match the Requirements for an Applicable and High-Throughput Sample Preparation Method from Biological Matrices?
Session Title: Proteomics (YOU 3)
Chairs: Norman Dovichi and Paige Malec
14:00 - Barry L. Karger: The Role of Separations in Protein and Proteomic Mass Spectrometric Analysis
14:40 - Adam Kecskemeti: Characterization of a Poly(dimethylsiloxane) Microfluidic Chip Containing Immobilized Trypsin for Rapid Protein Digestion
14:52 - Jana Zemenová: Novel Approach to Determine Lipidated Analogs of Prolactin-Releasing Peptide via LC-MS Using Monolithic Column
15:04 - Peng Yu: Trinity P1 Mixed Mode Chromatography Enables Mass Spectrometry Compatible Orthogonal Peptide Separation to Reversed Phase
15:16 - Elizaveta Solovyeva: Predictive Chromatography of Peptides for Optimized Fractionation in Bottom-Up Proteomics
Session Title: Stationary Phases Based on Inorganic Supports (FUN 4)
Chairs: Attila Felinger and Monika Dittman
16:30 - Joe Pesek: Aqueous Normal Phase Chromatography: The Reliable and Rugged Method for Polar Compound Analysis
17:00 - Frederic Lynen: Enhancing Peak Capacity in Temperature Responsive Liquid Chromatography
17:20 - Alla Chernobrovkina: Revealing the Trends in Selectivity Changes Altering the Functional Layer of Novel HILIC Stationary Phases
17:40 - Estrella Sanz Rodriguez: Porous Silica Layers Open Tubular Multi-Channel Capillaries as Micro Analytical Platforms for Separation and Extraction
Session Title: Multidimensional Chromatography and MS Coupling (HYP 4)
Chairs: Peter Schoenmakers and Mark Schure
16:30 - William Craig Byrdwell: Comprehensive Two-Dimensional Liquid Chromatography with Quadruple Parallel Mass Spectrometry, LC1MS2 x LC1MS2 = LC2MS4
17:00 - Dwight Stoll: Improving the Resolving Power and Detection Sensitivity of Two-Dimensional Liquid Chromatography Separations of Protein Digests
17:20 - Bert Wouters: Implementation of an Immobilised-Enzyme Microfluidic Reactor in an Integrated Multi-Dimensional Liquid Chromatography System
17:40 - C J Venkatramani: High Resolution Analysis of Linker Drugs Used in Antibody Drug Conjugates (ADC's) by 2D-LC-MS: Transition of 2D-LC-MS From Research to Main Stream Pharmaceutical Analysis
Session Title: Metabolomics (APP 4)
Chairs: Koji Otsuka and Rawi Ramautar
16:30 - Guowang Xu: Metabolomics: From Differential Metabolite Discovery to Functional Elucidation
17:00 - Liang Li: Comprehensive and Quantitative Profiling of the Human Blood Metabolome from One Microliter of Finger Blood
17:20 - Dajana Vuckovic: Improving Metabolite Coverage in Global Metabolomics Using Sequential Extraction
17:40 - Paige Malec: Derivatization Strategies for Targeted Metabolomics
Session Title: Sample Preparation (YOU 4)
Chairs: Janusz Pawliszyn and Gongke Li
16:30 - Boguslaw Buszewski: Similarity, Selectivity, and Specificity (3s) – Three Dimensional Factor Determining HPLC Resolution
17:10 - Andrew Quigley: Determination of Selected Fat Soluble Vitamins From Bovine Milk Using Dispersive Liquid-Liquid Microextraction Coupled with HPLC-UV Equipped with a Core-Shell Column
17:22 - Sara Tengattini: Development of an HPLC Bioreactor Based on Immobilized Enterokinase for the Cleavage of Fusion Proteins
17:34 - Michel Raetz: Automated Robotic Sample Preparation for Metabolomics with Hyphenation to LC-SWATH/MS Acquisition for Plasma and Liver Samples
17:46 - Deyber Arley Vargas Medina: Easy and Cheap Apparatus for the Off-Line Automatization of Miniaturized Sample Preparation Techniques
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.