DryLab®4
DryLab?4 offers users an intuitive and visual software solution for method development, optimization, trouble-shooting, robustness testing, and training. The new robustness module establishes the reliability of your method.
DryLab 4 is the next generation UHPLC modeling software. With its comprehensive resolution and robustness mapping abilities, DryLab 4 offers you an intuitive and visual software solution for method development, optimization, trouble-shooting, robustness testing, and training. DryLab 4’s 3D modeling capabilities and its multifactorial evaluation of flow rate, column dimensions, instrument parameters, and eluent composition in gradient and isocratic work, provide you with the tools to easily conform to Quality by Design standards.
The new Robustness Module establishes the reliability of your method in routine work by calculating how minor fluctuations in critical parameters affect the performance of your separation. Each parameter can be evaluated at 2 or 3 tolerance limits, and the success rate of the resulting 36 (=729) experiments can be measured in under 1 minute. An additional evaluation depicts which parameters exert the strongest influence on the method’s robustness, and tolerance limits can be individually varied to account for their corresponding impact. This testing process and analysis ensures that your finished method can withstand small inconsistencies in the parameter values of your set point and helps you to achieve a 100% success rate in routine applications, leading to inspection-safe methods.
Contact Details:
Molnár Institute for applied chromatography
Schneeglöckchenstraße 47, 10407 Berlin,
Germany
Email: info@molnar-institute.com
Phone: +49 30 421-5590
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.
