CE-TOFMS Analysis of Amino Acids
Researchers from Utrecht University?s (Utrecht, The Netherlands) Department of Biomedical Analysis have described a capillary electrophoresis?time-of-flight mass spectrometry (CE?TOFMS) method for analyzing amino acids in the urine of patients with urinary tract infections.
Researchers from Utrecht University's (Utrecht, The Netherlands) Department of Biomedical Analysis have described a capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) method for analyzing amino acids in the urine of patients with urinary tract infections. The method involves the use of capillaries coated with polybrene and poly(vinyl sulfonate) to obtain migration time repeatability with relative standard deviations of less than 2%. Limits of detection for the amino acids were reported to be as low as 20 nM. According to the researchers, the method shows good potential for the screening of body fluids based on the analysis of endogenous low molecular weight metabolites such as amino acids and related compounds.
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
